Experiment Overview
| Repository ID: | FR-FCM-ZZY9 | Experiment name: | Bleaching Response of Symbiodinium (Zooxanthellae) | MIFlowCyt score: | 48.50% |
| Primary researcher: | Co Sin Lee | PI/manager: | Co Sin Lee | Uploaded by: | Co Sin Lee |
| Experiment dates: | 2008-07-10 - 2011-12-09 | Dataset uploaded: | Apr 2012 | Last updated: | Jun 2013 |
| Keywords: | [bleaching response; zooxanthellae; chlorophyll; flow cytometry; microscopy] | Manuscripts: | [22865628] |
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| Organizations: |
National University of Singapore, Tropical Marine Science Institute, Singapore, (Singapore)
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| Purpose: | The experiment examined the effects of thermal stress on zooxanthellae densities and pigment degradation using flow cytometry. Potential applications of flow cytometry techniques to coral research are disccussed. | ||||
| Conclusion: | The importance of relative chlorophyll and β-carotene fluorescences were shown to be important in understanding the results of increased cell counts in freshly isolated zooxanthellae experimentally exposed to high temperatures (34, 36 and 38°C) over 24 hours, with ambient temperature (29°C) used as controls. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The results derived from FCM were validated against fluorescence microscopy. | ||||
