Experiment Overview
| Repository ID: | FR-FCM-ZZYE | Experiment name: | Functional performance of AmCyan100 in Flow Cytometry analysis | MIFlowCyt score: | 52.17% |
| Primary researcher: | Maria Jaimes | PI/manager: | Maria Jaimes | Uploaded by: | Maria Jaimes |
| Experiment dates: | 2012-02-01 - 2012-05-02 | Dataset uploaded: | May 2012 | Last updated: | Jan 2013 |
| Keywords: | [AmCyan100] [Stain Index] [Spillover] | Manuscripts: | [22674817] |
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| Organizations: |
BD Biosciences, Research and Development, San Jose, CA (USA)
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| Purpose: | A ? comparison of Stain Indices across CD4 (SK3) and CD8 (SK1) AmCyan, AmCyan100 and V500 B - comparison of spillover into other detectors (V450, FITC and PE) using cells stained with CD4 (SK3) AmCyan, AmCyan100 and V500. | ||||
| Conclusion: | The Stain Index of AmCyan100 CD4 and CD8 mAbs was similar to the one of the AmCyan and V500 reagents, indicating comparable sensitivity resolution/brightness. In addition, identical percentages of positive cells (data not shown) were detected across the 3 different CD4 or CD8 conjugates. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Stain Index for each antibody was measured in three different donors, | ||||
