Experiment Overview
| Repository ID: | FR-FCM-ZZYR | Experiment name: | User-defined protein marker assay development for characterization of circulating tumor cells using the CellSearch system | MIFlowCyt score: | 29.25% |
| Primary researcher: | Alison Allan | PI/manager: | Alison Allan | Uploaded by: | Alison Allan |
| Experiment dates: | 2008-10-01 - 2011-08-31 | Dataset uploaded: | Jul 2012 | Last updated: | Jan 2013 |
| Keywords: | [Cancer] [metastasis] [circulating tumor cells (CTCs)] [CD44] [M-30] [CellSearch® system] [user-defined marker] [CTC characterization] | Manuscripts: | [22899576] |
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| Organizations: |
London Regional Cancer Program, London Health Sciences Centre, Cancer Research, Flow Cytometry/Special Hematology, London, Ontario (Canada)
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| Purpose: | The purpose of the flow cytometry experiments in this study was to assess expression/presence of circulating tumor cell (CTC)-associated markers/characteristics in immortalized human cancer cell lines and normal human blood (i.e. percentage of cell population expressing the marker/characteristic of interest). | ||||
| Conclusion: | Flow cytometry analysis is a useful way of validating the percentage of cells within a given cell population that are positive for CTC-associated markers/characteristics of interest. This analysis provides a valuable starting point for optimization of user-defined markers for CTC characterization using the CellSearch® system (Veridex) | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | For marker expression analysis, FMO or appropriate IgG isotype controls were used. For experiments involving assessment of apoptosis, untreated cells were used as a negative control. | ||||
