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Experiment Overview

Repository ID: FR-FCM-ZZZA Experiment name: Human early preterm and full term cord blood study MIFlowCyt score: 70.50%
Primary researcher: Francesca D'Alessio PI/manager: Francesca D'Alessio Uploaded by: Francesca D'Alessio
Experiment dates: 2007-03-01 - 2010-07-26 Dataset uploaded: Aug 2011 Last updated: Jan 2013
Keywords: [Human early preterm cord blood] [CD34] [hematopoietic stem cells] Manuscripts: [21182179] Cytalogo
Organizations: Università degli Studi di Napoli Federico II, Napoli, N/A (Italy)
Purpose: The aim of our study was to compare the immunophenotype of human early preterm cord blood samples with these of human full term cord blood specimens. The first step was the detection and anlysis of the most immature CD34+CD38- population detected in two aforementioned kind of samples. Therefore we analyzed the frequency of the newly identified CD34+CD243+ subset in both early preterm and full term cord blood. In conclusion, we studied the maturation pattern of B-cells in the two kind of samples of our interest.
Conclusion: As to CD38, we observed that early preterm samples were richer in CD34+CD45dimCD38- immature cells compared to fullterm specimen. Our data showed that CD34+CD45dim cells from early preterm cord blood samples displayed higher percentage of CD133+CD38- undifferentiated cells respect tp full term counterparts. We therefore found a higher percentage of pro-B and pre-B cells in early preterm samples than in full term cord blood samples.
Comments: These results evidence the hematopoietic peculiarity of early preterm cord blood samples, potentially useful for developing novel strategies of stem cell-based therapy.
Funding: Not disclosed
Quality control: None
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