Experiment Overview
| Repository ID: | FR-FCM-ZY8E | Experiment name: | Aptamer dissociation constants | MIFlowCyt score: | 86.00% |
| Primary researcher: | Jennifer Soundy | PI/manager: | Darren Day | Uploaded by: | Jennifer Soundy |
| Experiment dates: | 2016-05-12 - 2016-10-27 | Dataset uploaded: | Jul 2017 | Last updated: | Jul 2017 |
| Keywords: | [dissociation kinetics] [Aptamer binding] [Pseudomonas aeruginosa] [SELEX progression] [FAM labelled aptamer] | Manuscripts: | |||
| Organizations: |
Victoria University of Wellington, School of Biological Sciences, Wellington, Wellington (New Zealand)
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| Purpose: | The purpose of these experiments was to determine the dissociation constants of the top aptamer candidates, the long, truncated and chimeric ones. Each aptamer was incubated with Pseudomonas at varying concentrations for 15 minutes then analysed by flow cytometry by looking for an increase in FAM fluorescence, indicating an increase in binding as the aptamers are FAM labelled. A dissociation constant was also done with the aptamers to E.coli as a non-specific association to minus from the specific binding seen on Pseudomonas. | ||||
| Conclusion: | Doing these experiments, and a lot of post flow analysis we were able to determine the dissociation constants of the aptamers. They were all in the low nanomolar range. | ||||
| Comments: | ALL KD FILES ARE IN THIS EXPERIMENT - I'VE DONE ALL THE ANALYSIS EXTERNALLY TO THIS PROGRAM. SEE ATTACHED FILES FOR AN EXAMPLE OF ANALYSIS. Analysis was done by gating the bacterial population in to two populations - bound and unbound. The unbound gate was drawn around the control cell population when displayed as FSC-A (Y) v FITC (X) dotplot. The bound gate was drawn around everything to the right of this on the X axis, so as the aptamer binds and fluorescence increases more bacteria will move in to the bound gate. These gates were then applied to each aptamer concentration file and the % pop total, % bound and % unbound were recorded. Numbers were adjusted relevant to total events = 100% and the % bound was plotted against concentration to make non-linear binding curves on graphpad prism 5.03. Kd values were determined using a specific one-site binding model, with E.coli measurements (% bound) being deducted as the non-specific binding values. Each aptamer Kd was measured in duplicate in two independent experiments and the results averaged across all of them (n=4) | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The same flow cytometer and settings are used for all experiments. The PBS used as flow buffer to re-suspend the bacteria in was run on its own to check for contamination or particulate that shows up as the same size as our target bacteria. | ||||
Experiment variables
| Doses | |
|---|---|
| · 1 nM | 08-17_1 nM_048.fcs · 17-21 rep 1_1 nM_003.fcs · 17-21 rep 1_1 nM_004.fcs · 17-21 rep 2_1 nM_011.fcs · 17-21 rep 2_1 nM_012.fcs · 17-21_1 nM_044.fcs · 21-17 rep 1_1 nM_003.fcs · 21-17 rep 1_1 nM_004.fcs · 21-17 rep 2_1 nM_011.fcs · 21-17 rep 2_1 nM_012.fcs · 21-17_1 nM_040.fcs · 8-17 rep 1_1 nM_003.fcs · 8-17 rep 1_1 nM_004.fcs · 8-17 rep 2_1 nM_011.fcs · 8-17 rep 2_1 nM_012.fcs · JN08 rep 1_1 nM_003.fcs · JN08 rep 1_1 nM_004.fcs · JN08 rep 2_1 nM_012.fcs · JN08 rep 2_1 nM_014.fcs · JN08-SH rep 1_1 nM_004.fcs · JN08-SH rep 1_1 nM_005.fcs · JN08-SH rep 2_1 nM_012.fcs · JN08-SH rep 2_1 nM_013.fcs · JN17-SH rep 1_1 nM_004.fcs · JN17-SH rep 1_1 nM_005.fcs · JN17-SH rep 2_1 nM_012.fcs · JN17-SH rep 2_1 nM_013.fcs · JN21-SH rep 1_1 nM_005.fcs · JN21-SH rep 1_1 nM_005.fcs-(1) · JN21-SH rep 2_1 nM_013.fcs · JN21-SH rep 2_1 nM_013.fcs-(1) · JN27 rep 1_1 nM_003.fcs · JN27 rep 1_1 nM_004.fcs · JN27 rep 2_1 nM_013.fcs · JN27 rep 2_1 nM_013.fcs-(1) · JN27-sh rep 1_1 nM_004.fcs · JN27-sh rep 1_1 nM_004.fcs-(1) · JN27-sh rep 2_1 nM_014.fcs · JN27-sh rep 2_1 nM_014.fcs-(1) · jn08-s_1 nM_016.fcs · jn08_1 nM_008.fcs · jn17-s_1 nM_028.fcs · jn21-s_1 nM_024.fcs · jn27-s_1 nM_020.fcs · jn27_1 nM_012.fcs |
| · 5 nM | 17-21 rep 1_5 nM_004.fcs · 17-21 rep 1_5 nM_005.fcs · 17-21 rep 2_5 nM_012.fcs · 17-21 rep 2_5 nM_013.fcs · 21-17 rep 1_5 nM_004.fcs · 21-17 rep 1_5 nM_005.fcs · 21-17 rep 2_5 nM_012.fcs · 21-17 rep 2_5 nM_013.fcs · 8-17 rep 1_5 nM_004.fcs · 8-17 rep 1_5 nM_005.fcs · 8-17 rep 2_5 nM_012.fcs · 8-17 rep 2_5 nM_013.fcs · JN08 rep 1_5 nM_004.fcs · JN08 rep 1_5 nM_005.fcs · JN08 rep 2_5 nM_013.fcs · JN08 rep 2_5 nM_015.fcs · JN08-SH rep 1_5 nM_005.fcs · JN08-SH rep 1_5 nM_006.fcs · JN08-SH rep 2_5 nM_013.fcs · JN08-SH rep 2_5 nM_014.fcs · JN17-SH rep 1_5 nM_005.fcs · JN17-SH rep 1_5 nM_006.fcs · JN17-SH rep 2_5 nM_013.fcs · JN17-SH rep 2_5 nM_014.fcs · JN21-SH rep 1_5 nM_006.fcs · JN21-SH rep 1_5 nM_006.fcs-(1) · JN21-SH rep 2_5 nM_014.fcs · JN21-SH rep 2_5 nM_014.fcs-(1) · JN27 rep 1_5 nM_004.fcs · JN27 rep 1_5 nM_005.fcs · JN27 rep 2_5 nM_014.fcs · JN27 rep 2_5 nM_014.fcs-(1) · JN27-sh rep 1_5 nM_005.fcs · JN27-sh rep 1_5 nM_005.fcs-(1) · JN27-sh rep 2_5 nM_015.fcs · JN27-sh rep 2_5 nM_015.fcs-(1) |
| · 10 nM | 08-17_10 nM_049.fcs · 17-21 rep 1_10 nM_005.fcs · 17-21 rep 1_10 nM_006.fcs · 17-21 rep 2_10 nM_013.fcs · 17-21 rep 2_10 nM_014.fcs · 17-21_10 nM_045.fcs · 21-17 rep 1_10 nM_005.fcs · 21-17 rep 1_10 nM_006.fcs · 21-17 rep 2_10 nM_013.fcs · 21-17 rep 2_10 nM_014.fcs · 21-17_10 nM_041.fcs · 8-17 rep 1_10 nM_005.fcs · 8-17 rep 1_10 nM_006.fcs · 8-17 rep 2_10 nM_013.fcs · 8-17 rep 2_10 nM_014.fcs · JN08 rep 1_10 nM_005.fcs · JN08 rep 1_10 nM_006.fcs · JN08 rep 2_10 nM_014.fcs · JN08 rep 2_10 nM_016.fcs · JN08-SH rep 1_10 nM_006.fcs · JN08-SH rep 1_10 nM_007.fcs · JN08-SH rep 2_10 nM_014.fcs · JN08-SH rep 2_10 nM_015.fcs · JN17-SH rep 1_10 nM_006.fcs · JN17-SH rep 1_10 nM_007.fcs · JN17-SH rep 2_10 nM_014.fcs · JN17-SH rep 2_10 nM_015.fcs · JN21-SH rep 1_10 nM_007.fcs · JN21-SH rep 1_10 nM_007.fcs-(1) · JN21-SH rep 2_10 nM_015.fcs · JN21-SH rep 2_10 nM_015.fcs-(1) · JN27 rep 1_10 nM_005.fcs · JN27 rep 1_10 nM_006.fcs · JN27 rep 2_10 nM_015.fcs · JN27 rep 2_10 nM_015.fcs-(1) · JN27-sh rep 1_10 nM_006.fcs · JN27-sh rep 1_10 nM_006.fcs-(1) · JN27-sh rep 2_10 nM_016.fcs · JN27-sh rep 2_10 nM_016.fcs-(1) · jn08-s_10 nM_017.fcs · jn08_10 nM_009.fcs · jn17-s_10 nM_029.fcs · jn21-s_10 nM_025.fcs · jn27-s_10 nM_021.fcs · jn27_10 nM_013.fcs |
| · 20 nM | 17-21 rep 1_20 nM_006.fcs · 17-21 rep 1_20 nM_007.fcs · 17-21 rep 2_20 nM_014.fcs · 17-21 rep 2_20 nM_015.fcs · 21-17 rep 1_20 nM_006.fcs · 21-17 rep 1_20 nM_007.fcs · 21-17 rep 2_20 nM_014.fcs · 21-17 rep 2_20 nM_015.fcs · 8-17 rep 1_20 nM_006.fcs · 8-17 rep 1_20 nM_007.fcs · 8-17 rep 2_20 nM_014.fcs · 8-17 rep 2_20 nM_015.fcs · JN08 rep 1_20 nM_006.fcs · JN08 rep 1_20 nM_007.fcs · JN08 rep 2_20 nM_015.fcs · JN08 rep 2_20 nM_017.fcs · JN08-SH rep 1_20 nM_007.fcs · JN08-SH rep 1_20 nM_008.fcs · JN08-SH rep 2_20 nM_015.fcs · JN08-SH rep 2_20 nM_016.fcs · JN17-SH rep 1_20 nM_007.fcs · JN17-SH rep 1_20 nM_008.fcs · JN17-SH rep 2_20 nM_015.fcs · JN17-SH rep 2_20 nM_016.fcs · JN21-SH rep 1_20 nM_008.fcs · JN21-SH rep 1_20 nM_008.fcs-(1) · JN21-SH rep 2_20 nM_016.fcs · JN21-SH rep 2_20 nM_016.fcs-(1) · JN27 rep 1_20 nM_006.fcs · JN27 rep 1_20 nM_007.fcs · JN27 rep 2_20 nM_016.fcs · JN27 rep 2_20 nM_016.fcs-(1) · JN27-sh rep 1_20 nM_007.fcs · JN27-sh rep 1_20 nM_007.fcs-(1) · JN27-sh rep 2_20 nM_017.fcs · JN27-sh rep 2_20 nM_017.fcs-(1) |
| · 30 nM | 17-21 rep 1_30 nM_007.fcs · 17-21 rep 1_30 nM_008.fcs · 17-21 rep 2_30 nM_015.fcs · 17-21 rep 2_30 nM_016.fcs · 21-17 rep 1_30 nM_007.fcs · 21-17 rep 1_30 nM_008.fcs · 21-17 rep 2_30 nM_015.fcs · 21-17 rep 2_30 nM_016.fcs · 8-17 rep 1_30 nM_007.fcs · 8-17 rep 1_30 nM_008.fcs · 8-17 rep 2_30 nM_015.fcs · 8-17 rep 2_30 nM_016.fcs · JN08 rep 1_30 nM_007.fcs · JN08 rep 1_30 nM_008.fcs · JN08 rep 2_30 nM_016.fcs · JN08 rep 2_30 nM_018.fcs · JN08-SH rep 1_30 nM_008.fcs · JN08-SH rep 1_30 nM_009.fcs · JN08-SH rep 2_30 nM_016.fcs · JN08-SH rep 2_30 nM_017.fcs · JN17-SH rep 1_30 nM_008.fcs · JN17-SH rep 1_30 nM_009.fcs · JN17-SH rep 2_30 nM_016.fcs · JN17-SH rep 2_30 nM_017.fcs · JN21-SH rep 1_30 nM_009.fcs · JN21-SH rep 1_30 nM_009.fcs-(1) · JN21-SH rep 2_30 nM_017.fcs · JN21-SH rep 2_30 nM_017.fcs-(1) · JN27 rep 1_30 nM_007.fcs · JN27 rep 1_30 nM_008.fcs · JN27 rep 2_30 nM_017.fcs · JN27 rep 2_30 nM_017.fcs-(1) · JN27-sh rep 1_30 nM_008.fcs · JN27-sh rep 1_30 nM_008.fcs-(1) · JN27-sh rep 2_30 nM_018.fcs · JN27-sh rep 2_30 nM_018.fcs-(1) |
| · 50 nM | 08-17_50 nM_050.fcs · 17-21 rep 1_50 nM_008.fcs · 17-21 rep 1_50 nM_009.fcs · 17-21 rep 2_50 nM_016.fcs · 17-21 rep 2_50 nM_017.fcs · 17-21_50 nM_046.fcs · 21-17 rep 1_50 nM_008.fcs · 21-17 rep 1_50 nM_009.fcs · 21-17 rep 2_50 nM_016.fcs · 21-17 rep 2_50 nM_017.fcs · 21-17_50 nM_042.fcs · 8-17 rep 1_50 nM_008.fcs · 8-17 rep 1_50 nM_009.fcs · 8-17 rep 2_50 nM_016.fcs · 8-17 rep 2_50 nM_017.fcs · JN08 rep 1_50 nM_008.fcs · JN08 rep 1_50 nM_009.fcs · JN08 rep 2_50 nM_017.fcs · JN08 rep 2_50 nM_019.fcs · JN08-SH rep 1_50 nM_009.fcs · JN08-SH rep 1_50 nM_010.fcs · JN08-SH rep 2_50 nM_017.fcs · JN08-SH rep 2_50 nM_018.fcs · JN17-SH rep 1_50 nM_009.fcs · JN17-SH rep 1_50 nM_010.fcs · JN17-SH rep 2_50 nM_017.fcs · JN17-SH rep 2_50 nM_018.fcs · JN21-SH rep 1_50 nM_010.fcs · JN21-SH rep 1_50 nM_010.fcs-(1) · JN21-SH rep 2_50 nM_018.fcs · JN21-SH rep 2_50 nM_018.fcs-(1) · JN27 rep 1_50 nM_008.fcs · JN27 rep 1_50 nM_009.fcs · JN27 rep 2_50 nM_018.fcs · JN27 rep 2_50 nM_018.fcs-(1) · JN27-sh rep 1_50 nM_009.fcs · JN27-sh rep 1_50 nM_009.fcs-(1) · JN27-sh rep 2_50 nM_019.fcs · JN27-sh rep 2_50 nM_019.fcs-(1) · jn08-s_50 nM_018.fcs · jn08_50 nM_010.fcs · jn17-s_50 nM_030.fcs · jn21-s_50 nM_026.fcs · jn27-s_50 nM_022.fcs · jn27_50 nM_014.fcs |
| · 100 nM | 17-21 rep 1_100 nM_009.fcs · 17-21 rep 1_100 nM_010.fcs · 17-21 rep 2_100 nM_017.fcs · 17-21 rep 2_100 nM_018.fcs · 21-17 rep 1_100 nM_009.fcs · 21-17 rep 1_100 nM_010.fcs · 21-17 rep 2_100 nM_017.fcs · 21-17 rep 2_100 nM_018.fcs · 8-17 rep 1_100 nM_009.fcs · 8-17 rep 1_100 nM_010.fcs · 8-17 rep 2_100 nM_017.fcs · 8-17 rep 2_100 nM_018.fcs · JN08 rep 1_100 nM_009.fcs · JN08 rep 1_100 nM_010.fcs · JN08 rep 2_100 nM_018.fcs · JN08 rep 2_100 nM_020.fcs · JN08-SH rep 1_100 nM_010.fcs · JN08-SH rep 1_100 nM_011.fcs · JN08-SH rep 2_100 nM_018.fcs · JN08-SH rep 2_100 nM_019.fcs · JN17-SH rep 1_100 nM_010.fcs · JN17-SH rep 1_100 nM_011.fcs · JN17-SH rep 2_100 nM_018.fcs · JN17-SH rep 2_100 nM_019.fcs · JN21-SH rep 1_100 nM_011.fcs · JN21-SH rep 1_100 nM_011.fcs-(1) · JN21-SH rep 2_100 nM_019.fcs · JN27 rep 1_100 nM_009.fcs · JN27 rep 1_100 nM_010.fcs · JN27 rep 2_100 nM_019.fcs · JN27 rep 2_100 nM_019.fcs-(1) · JN27-sh rep 1_100 nM_010.fcs · JN27-sh rep 1_100 nM_010.fcs-(1) · JN27-sh rep 2_100 nM_020.fcs · JN27-sh rep 2_100 nM_020.fcs-(1) |
| · 250 nM | 08-17_250 nM_051.fcs · 17-21 rep 1_250 nM_010.fcs · 17-21 rep 1_250 nM_011.fcs · 17-21 rep 2_250 nM_018.fcs · 17-21 rep 2_250 nM_019.fcs · 17-21_250 nM_047.fcs · 21-17 rep 1_250 nM_010.fcs · 21-17 rep 1_250 nM_011.fcs · 21-17 rep 2_250 nM_018.fcs · 21-17 rep 2_250 nM_019.fcs · 21-17_250 nM_043.fcs · 8-17 rep 1_250 nM_010.fcs · 8-17 rep 1_250 nM_011.fcs · 8-17 rep 2_250 nM_018.fcs · 8-17 rep 2_250 nM_019.fcs · JN08 rep 1_250 nM_010.fcs · JN08 rep 1_250 nM_011.fcs · JN08 rep 2_250 nM_019.fcs · JN08 rep 2_250 nM_021.fcs · JN08-SH rep 1_250 nM_011.fcs · JN08-SH rep 1_250 nM_012.fcs · JN08-SH rep 2_250 nM_019.fcs · JN08-SH rep 2_250 nM_020.fcs · JN17-SH rep 1_250 nM_011.fcs · JN17-SH rep 1_250 nM_012.fcs · JN17-SH rep 2_250 nM_019.fcs · JN17-SH rep 2_250 nM_020.fcs · JN21-SH rep 1_250 nM_012.fcs · JN21-SH rep 1_250 nM_012.fcs-(1) · JN21-SH rep 2_250 nM_020.fcs · JN21-SH rep 2_250 nM_020.fcs-(1) · JN27 rep 1_250 nM_010.fcs · JN27 rep 1_250 nM_011.fcs · JN27 rep 2_250 nM_020.fcs · JN27 rep 2_250 nM_020.fcs-(1) · JN27-sh rep 1_250 nM_011.fcs · JN27-sh rep 1_250 nM_011.fcs-(1) · JN27-sh rep 2_250 nM_021.fcs · JN27-sh rep 2_250 nM_021.fcs-(1) · jn08-s_250 nM_019.fcs · jn08_250 nM_011.fcs · jn17-s_250 nM_031.fcs · jn21-s_250 nM_027.fcs · jn27-s_250 nM_023.fcs · jn27_250 nM_015.fcs |
| · No treatment | Control cells_PA692 2_002.fcs · Control cells_PA692 2_002.fcs-(1) · Control cells_PA692 2_002.fcs-(2) · Control cells_PA692 2_003.fcs · Control cells_PA692 2_003.fcs-(1) · Control cells_PA692 2_003.fcs-(2) · Control cells_PA692_001.fcs · Control cells_PA692_001.fcs-(1) · Control cells_PA692_001.fcs-(2) · Control cells_PA692_001.fcs-(3) · Control cells_PA692_001.fcs-(4) · Control cells_PA692_002.fcs · Control cells_PA692_002.fcs-(1) · Control cells_PA692_002.fcs-(2) · Control cells_PA692_002.fcs-(3) · Control cells_PA692_002.fcs-(4) · Control cells_PA692_002.fcs-(5) · Control cells_PA692_002.fcs-(6) · Control cells_PA692_002.fcs-(7) · Control cells_PBS_001.fcs · Control cells_PBS_001.fcs-(1) · Control cells_PBS_001.fcs-(10) · Control cells_PBS_001.fcs-(2) · Control cells_PBS_001.fcs-(3) · Control cells_PBS_001.fcs-(4) · Control cells_PBS_001.fcs-(5) · Control cells_PBS_001.fcs-(6) · Control cells_PBS_001.fcs-(7) · Control cells_PBS_001.fcs-(8) · Control cells_PBS_001.fcs-(9) · Control cells_ecoli 6_005.fcs |
| · 500 nM | JN08 rep 1_500 nM_011.fcs · JN08 rep 1_500 nM_012.fcs · JN08 rep 2_500 nM_020.fcs · JN08 rep 2_500 nM_022.fcs · JN27 rep 1_500 nM_011.fcs · JN27 rep 1_500 nM_012.fcs · JN27 rep 2_500 nM_021.fcs · JN27 rep 2_500 nM_021.fcs-(1) · JN27-sh rep 1_500 nM_012.fcs · JN27-sh rep 1_500 nM_012.fcs-(1) · JN27-sh rep 2_500 nM_022.fcs · JN27-sh rep 2_500 nM_022.fcs-(1) |
| · 750 nM | JN08 rep 1_750 nM_013.fcs · JN08 rep 2_750 nM_023.fcs · JN27 rep 1_750 nM_012.fcs · JN27 rep 2_750 nM_022.fcs · JN27-sh rep 1_750 nM_013.fcs · JN27-sh rep 1_750 nM_013.fcs-(1) · JN27-sh rep 2_750 nM_023.fcs · JN27-sh rep 2_750 nM_023.fcs-(1) |
| Conditions | |
|---|---|
| · JN21.SH | JN21-SH rep 1_1 nM_005.fcs · JN21-SH rep 1_1 nM_005.fcs-(1) · JN21-SH rep 1_10 nM_007.fcs · JN21-SH rep 1_10 nM_007.fcs-(1) · JN21-SH rep 1_100 nM_011.fcs · JN21-SH rep 1_100 nM_011.fcs-(1) · JN21-SH rep 1_20 nM_008.fcs · JN21-SH rep 1_20 nM_008.fcs-(1) · JN21-SH rep 1_250 nM_012.fcs · JN21-SH rep 1_250 nM_012.fcs-(1) · JN21-SH rep 1_30 nM_009.fcs · JN21-SH rep 1_30 nM_009.fcs-(1) · JN21-SH rep 1_5 nM_006.fcs · JN21-SH rep 1_5 nM_006.fcs-(1) · JN21-SH rep 1_50 nM_010.fcs · JN21-SH rep 1_50 nM_010.fcs-(1) · JN21-SH rep 2_1 nM_013.fcs · JN21-SH rep 2_1 nM_013.fcs-(1) · JN21-SH rep 2_10 nM_015.fcs · JN21-SH rep 2_10 nM_015.fcs-(1) · JN21-SH rep 2_100 nM_019.fcs · JN21-SH rep 2_20 nM_016.fcs · JN21-SH rep 2_20 nM_016.fcs-(1) · JN21-SH rep 2_250 nM_020.fcs · JN21-SH rep 2_250 nM_020.fcs-(1) · JN21-SH rep 2_30 nM_017.fcs · JN21-SH rep 2_30 nM_017.fcs-(1) · JN21-SH rep 2_5 nM_014.fcs · JN21-SH rep 2_5 nM_014.fcs-(1) · JN21-SH rep 2_50 nM_018.fcs · JN21-SH rep 2_50 nM_018.fcs-(1) · jn21-s_1 nM_024.fcs · jn21-s_10 nM_025.fcs · jn21-s_250 nM_027.fcs · jn21-s_50 nM_026.fcs |
| · St21Lp17 | 21-17 rep 1_1 nM_003.fcs · 21-17 rep 1_1 nM_004.fcs · 21-17 rep 1_10 nM_005.fcs · 21-17 rep 1_10 nM_006.fcs · 21-17 rep 1_100 nM_009.fcs · 21-17 rep 1_100 nM_010.fcs · 21-17 rep 1_20 nM_006.fcs · 21-17 rep 1_20 nM_007.fcs · 21-17 rep 1_250 nM_010.fcs · 21-17 rep 1_250 nM_011.fcs · 21-17 rep 1_30 nM_007.fcs · 21-17 rep 1_30 nM_008.fcs · 21-17 rep 1_5 nM_004.fcs · 21-17 rep 1_5 nM_005.fcs · 21-17 rep 1_50 nM_008.fcs · 21-17 rep 1_50 nM_009.fcs · 21-17 rep 2_1 nM_011.fcs · 21-17 rep 2_1 nM_012.fcs · 21-17 rep 2_10 nM_013.fcs · 21-17 rep 2_10 nM_014.fcs · 21-17 rep 2_100 nM_017.fcs · 21-17 rep 2_100 nM_018.fcs · 21-17 rep 2_20 nM_014.fcs · 21-17 rep 2_20 nM_015.fcs · 21-17 rep 2_250 nM_018.fcs · 21-17 rep 2_250 nM_019.fcs · 21-17 rep 2_30 nM_015.fcs · 21-17 rep 2_30 nM_016.fcs · 21-17 rep 2_5 nM_012.fcs · 21-17 rep 2_5 nM_013.fcs · 21-17 rep 2_50 nM_016.fcs · 21-17 rep 2_50 nM_017.fcs · 21-17_1 nM_040.fcs · 21-17_10 nM_041.fcs · 21-17_250 nM_043.fcs · 21-17_50 nM_042.fcs |
| · St17lp21 | 17-21 rep 1_1 nM_003.fcs · 17-21 rep 1_1 nM_004.fcs · 17-21 rep 1_10 nM_005.fcs · 17-21 rep 1_10 nM_006.fcs · 17-21 rep 1_100 nM_009.fcs · 17-21 rep 1_100 nM_010.fcs · 17-21 rep 1_20 nM_006.fcs · 17-21 rep 1_20 nM_007.fcs · 17-21 rep 1_250 nM_010.fcs · 17-21 rep 1_250 nM_011.fcs · 17-21 rep 1_30 nM_007.fcs · 17-21 rep 1_30 nM_008.fcs · 17-21 rep 1_5 nM_004.fcs · 17-21 rep 1_5 nM_005.fcs · 17-21 rep 1_50 nM_008.fcs · 17-21 rep 1_50 nM_009.fcs · 17-21 rep 2_1 nM_011.fcs · 17-21 rep 2_1 nM_012.fcs · 17-21 rep 2_10 nM_013.fcs · 17-21 rep 2_10 nM_014.fcs · 17-21 rep 2_100 nM_017.fcs · 17-21 rep 2_100 nM_018.fcs · 17-21 rep 2_20 nM_014.fcs · 17-21 rep 2_20 nM_015.fcs · 17-21 rep 2_250 nM_018.fcs · 17-21 rep 2_250 nM_019.fcs · 17-21 rep 2_30 nM_015.fcs · 17-21 rep 2_30 nM_016.fcs · 17-21 rep 2_5 nM_012.fcs · 17-21 rep 2_5 nM_013.fcs · 17-21 rep 2_50 nM_016.fcs · 17-21 rep 2_50 nM_017.fcs · 17-21_1 nM_044.fcs · 17-21_10 nM_045.fcs · 17-21_250 nM_047.fcs · 17-21_50 nM_046.fcs |
| · JN08.SH | JN08-SH rep 1_1 nM_004.fcs · JN08-SH rep 1_1 nM_005.fcs · JN08-SH rep 1_10 nM_006.fcs · JN08-SH rep 1_10 nM_007.fcs · JN08-SH rep 1_100 nM_010.fcs · JN08-SH rep 1_100 nM_011.fcs · JN08-SH rep 1_20 nM_007.fcs · JN08-SH rep 1_20 nM_008.fcs · JN08-SH rep 1_250 nM_011.fcs · JN08-SH rep 1_250 nM_012.fcs · JN08-SH rep 1_30 nM_008.fcs · JN08-SH rep 1_30 nM_009.fcs · JN08-SH rep 1_5 nM_005.fcs · JN08-SH rep 1_5 nM_006.fcs · JN08-SH rep 1_50 nM_009.fcs · JN08-SH rep 1_50 nM_010.fcs · JN08-SH rep 2_1 nM_012.fcs · JN08-SH rep 2_1 nM_013.fcs · JN08-SH rep 2_10 nM_014.fcs · JN08-SH rep 2_10 nM_015.fcs · JN08-SH rep 2_100 nM_018.fcs · JN08-SH rep 2_100 nM_019.fcs · JN08-SH rep 2_20 nM_015.fcs · JN08-SH rep 2_20 nM_016.fcs · JN08-SH rep 2_250 nM_019.fcs · JN08-SH rep 2_250 nM_020.fcs · JN08-SH rep 2_30 nM_016.fcs · JN08-SH rep 2_30 nM_017.fcs · JN08-SH rep 2_5 nM_013.fcs · JN08-SH rep 2_5 nM_014.fcs · JN08-SH rep 2_50 nM_017.fcs · JN08-SH rep 2_50 nM_018.fcs · jn08-s_1 nM_016.fcs · jn08-s_10 nM_017.fcs · jn08-s_250 nM_019.fcs · jn08-s_50 nM_018.fcs · jn08_1 nM_008.fcs · jn08_10 nM_009.fcs · jn08_250 nM_011.fcs · jn08_50 nM_010.fcs |
| · JN27.SH | JN27-sh rep 1_1 nM_004.fcs · JN27-sh rep 1_1 nM_004.fcs-(1) · JN27-sh rep 1_10 nM_006.fcs · JN27-sh rep 1_10 nM_006.fcs-(1) · JN27-sh rep 1_100 nM_010.fcs · JN27-sh rep 1_100 nM_010.fcs-(1) · JN27-sh rep 1_20 nM_007.fcs · JN27-sh rep 1_20 nM_007.fcs-(1) · JN27-sh rep 1_250 nM_011.fcs · JN27-sh rep 1_250 nM_011.fcs-(1) · JN27-sh rep 1_30 nM_008.fcs · JN27-sh rep 1_30 nM_008.fcs-(1) · JN27-sh rep 1_5 nM_005.fcs · JN27-sh rep 1_5 nM_005.fcs-(1) · JN27-sh rep 1_50 nM_009.fcs · JN27-sh rep 1_50 nM_009.fcs-(1) · JN27-sh rep 1_500 nM_012.fcs · JN27-sh rep 1_500 nM_012.fcs-(1) · JN27-sh rep 1_750 nM_013.fcs · JN27-sh rep 1_750 nM_013.fcs-(1) · JN27-sh rep 2_1 nM_014.fcs · JN27-sh rep 2_1 nM_014.fcs-(1) · JN27-sh rep 2_10 nM_016.fcs · JN27-sh rep 2_10 nM_016.fcs-(1) · JN27-sh rep 2_100 nM_020.fcs · JN27-sh rep 2_100 nM_020.fcs-(1) · JN27-sh rep 2_20 nM_017.fcs · JN27-sh rep 2_20 nM_017.fcs-(1) · JN27-sh rep 2_250 nM_021.fcs · JN27-sh rep 2_250 nM_021.fcs-(1) · JN27-sh rep 2_30 nM_018.fcs · JN27-sh rep 2_30 nM_018.fcs-(1) · JN27-sh rep 2_5 nM_015.fcs · JN27-sh rep 2_5 nM_015.fcs-(1) · JN27-sh rep 2_50 nM_019.fcs · JN27-sh rep 2_50 nM_019.fcs-(1) · JN27-sh rep 2_500 nM_022.fcs · JN27-sh rep 2_500 nM_022.fcs-(1) · JN27-sh rep 2_750 nM_023.fcs · JN27-sh rep 2_750 nM_023.fcs-(1) · jn27-s_1 nM_020.fcs · jn27-s_10 nM_021.fcs · jn27-s_250 nM_023.fcs · jn27-s_50 nM_022.fcs · jn27_1 nM_012.fcs · jn27_10 nM_013.fcs · jn27_250 nM_015.fcs · jn27_50 nM_014.fcs |
| · JN08 | JN08 rep 1_1 nM_003.fcs · JN08 rep 1_1 nM_004.fcs · JN08 rep 1_10 nM_005.fcs · JN08 rep 1_10 nM_006.fcs · JN08 rep 1_100 nM_009.fcs · JN08 rep 1_100 nM_010.fcs · JN08 rep 1_20 nM_006.fcs · JN08 rep 1_20 nM_007.fcs · JN08 rep 1_250 nM_010.fcs · JN08 rep 1_250 nM_011.fcs · JN08 rep 1_30 nM_007.fcs · JN08 rep 1_30 nM_008.fcs · JN08 rep 1_5 nM_004.fcs · JN08 rep 1_5 nM_005.fcs · JN08 rep 1_50 nM_008.fcs · JN08 rep 1_50 nM_009.fcs · JN08 rep 1_500 nM_011.fcs · JN08 rep 1_500 nM_012.fcs · JN08 rep 1_750 nM_013.fcs · JN08 rep 2_1 nM_012.fcs · JN08 rep 2_1 nM_014.fcs · JN08 rep 2_10 nM_014.fcs · JN08 rep 2_10 nM_016.fcs · JN08 rep 2_100 nM_018.fcs · JN08 rep 2_100 nM_020.fcs · JN08 rep 2_20 nM_015.fcs · JN08 rep 2_20 nM_017.fcs · JN08 rep 2_250 nM_019.fcs · JN08 rep 2_250 nM_021.fcs · JN08 rep 2_30 nM_016.fcs · JN08 rep 2_30 nM_018.fcs · JN08 rep 2_5 nM_013.fcs · JN08 rep 2_5 nM_015.fcs · JN08 rep 2_50 nM_017.fcs · JN08 rep 2_50 nM_019.fcs · JN08 rep 2_500 nM_020.fcs · JN08 rep 2_500 nM_022.fcs · JN08 rep 2_750 nM_023.fcs |
| · JN27 | JN27 rep 1_1 nM_003.fcs · JN27 rep 1_1 nM_004.fcs · JN27 rep 1_10 nM_005.fcs · JN27 rep 1_10 nM_006.fcs · JN27 rep 1_100 nM_009.fcs · JN27 rep 1_100 nM_010.fcs · JN27 rep 1_20 nM_006.fcs · JN27 rep 1_20 nM_007.fcs · JN27 rep 1_250 nM_010.fcs · JN27 rep 1_250 nM_011.fcs · JN27 rep 1_30 nM_007.fcs · JN27 rep 1_30 nM_008.fcs · JN27 rep 1_5 nM_004.fcs · JN27 rep 1_5 nM_005.fcs · JN27 rep 1_50 nM_008.fcs · JN27 rep 1_50 nM_009.fcs · JN27 rep 1_500 nM_011.fcs · JN27 rep 1_500 nM_012.fcs · JN27 rep 1_750 nM_012.fcs · JN27 rep 2_1 nM_013.fcs · JN27 rep 2_1 nM_013.fcs-(1) · JN27 rep 2_10 nM_015.fcs · JN27 rep 2_10 nM_015.fcs-(1) · JN27 rep 2_100 nM_019.fcs · JN27 rep 2_100 nM_019.fcs-(1) · JN27 rep 2_20 nM_016.fcs · JN27 rep 2_20 nM_016.fcs-(1) · JN27 rep 2_250 nM_020.fcs · JN27 rep 2_250 nM_020.fcs-(1) · JN27 rep 2_30 nM_017.fcs · JN27 rep 2_30 nM_017.fcs-(1) · JN27 rep 2_5 nM_014.fcs · JN27 rep 2_5 nM_014.fcs-(1) · JN27 rep 2_50 nM_018.fcs · JN27 rep 2_50 nM_018.fcs-(1) · JN27 rep 2_500 nM_021.fcs · JN27 rep 2_500 nM_021.fcs-(1) · JN27 rep 2_750 nM_022.fcs |
| · JN17.SH | JN17-SH rep 1_1 nM_004.fcs · JN17-SH rep 1_1 nM_005.fcs · JN17-SH rep 1_10 nM_006.fcs · JN17-SH rep 1_10 nM_007.fcs · JN17-SH rep 1_100 nM_010.fcs · JN17-SH rep 1_100 nM_011.fcs · JN17-SH rep 1_20 nM_007.fcs · JN17-SH rep 1_20 nM_008.fcs · JN17-SH rep 1_250 nM_011.fcs · JN17-SH rep 1_250 nM_012.fcs · JN17-SH rep 1_30 nM_008.fcs · JN17-SH rep 1_30 nM_009.fcs · JN17-SH rep 1_5 nM_005.fcs · JN17-SH rep 1_5 nM_006.fcs · JN17-SH rep 1_50 nM_009.fcs · JN17-SH rep 1_50 nM_010.fcs · JN17-SH rep 2_1 nM_012.fcs · JN17-SH rep 2_1 nM_013.fcs · JN17-SH rep 2_10 nM_014.fcs · JN17-SH rep 2_10 nM_015.fcs · JN17-SH rep 2_100 nM_018.fcs · JN17-SH rep 2_100 nM_019.fcs · JN17-SH rep 2_20 nM_015.fcs · JN17-SH rep 2_20 nM_016.fcs · JN17-SH rep 2_250 nM_019.fcs · JN17-SH rep 2_250 nM_020.fcs · JN17-SH rep 2_30 nM_016.fcs · JN17-SH rep 2_30 nM_017.fcs · JN17-SH rep 2_5 nM_013.fcs · JN17-SH rep 2_5 nM_014.fcs · JN17-SH rep 2_50 nM_017.fcs · JN17-SH rep 2_50 nM_018.fcs · jn17-s_1 nM_028.fcs · jn17-s_10 nM_029.fcs · jn17-s_250 nM_031.fcs · jn17-s_50 nM_030.fcs |
| · St08Lp17 | 08-17_1 nM_048.fcs · 08-17_10 nM_049.fcs · 08-17_250 nM_051.fcs · 08-17_50 nM_050.fcs · 8-17 rep 1_1 nM_003.fcs · 8-17 rep 1_1 nM_004.fcs · 8-17 rep 1_10 nM_005.fcs · 8-17 rep 1_10 nM_006.fcs · 8-17 rep 1_100 nM_009.fcs · 8-17 rep 1_100 nM_010.fcs · 8-17 rep 1_20 nM_006.fcs · 8-17 rep 1_20 nM_007.fcs · 8-17 rep 1_250 nM_010.fcs · 8-17 rep 1_250 nM_011.fcs · 8-17 rep 1_30 nM_007.fcs · 8-17 rep 1_30 nM_008.fcs · 8-17 rep 1_5 nM_004.fcs · 8-17 rep 1_5 nM_005.fcs · 8-17 rep 1_50 nM_008.fcs · 8-17 rep 1_50 nM_009.fcs · 8-17 rep 2_1 nM_011.fcs · 8-17 rep 2_1 nM_012.fcs · 8-17 rep 2_10 nM_013.fcs · 8-17 rep 2_10 nM_014.fcs · 8-17 rep 2_100 nM_017.fcs · 8-17 rep 2_100 nM_018.fcs · 8-17 rep 2_20 nM_014.fcs · 8-17 rep 2_20 nM_015.fcs · 8-17 rep 2_250 nM_018.fcs · 8-17 rep 2_250 nM_019.fcs · 8-17 rep 2_30 nM_015.fcs · 8-17 rep 2_30 nM_016.fcs · 8-17 rep 2_5 nM_012.fcs · 8-17 rep 2_5 nM_013.fcs · 8-17 rep 2_50 nM_016.fcs · 8-17 rep 2_50 nM_017.fcs |
