Experiment Overview
| Repository ID: | FR-FCM-Z5FM | Experiment name: | Lungs mix 2 Male vs female naive and GBS 24h | MIFlowCyt score: | 50.00% |
| Primary researcher: | Maud Deny | PI/manager: | Maud Deny | Uploaded by: | Maud Deny |
| Experiment dates: | 2019-03-19 - | Dataset uploaded: | Jun 2022 | Last updated: | Jun 2022 |
| Keywords: | [mice] [granulocytes] [GBS] [lungs] | Manuscripts: | |||
| Organizations: |
Inflammation Unit, Laboratory of Pediatric Research, ULB, Brussels, (Belgium)
Immune Response Service, Sciensano, Brussels, (Belgium) |
||||
| Purpose: | This experiment was performed to compare pulmonary granulocytes (neutrophils and eosinophils) between 4-5 week old male and female C57BL/6 mice, naive or 24 hours after intranasal infection with 1.10^8 CFU of group B streptococcus. | ||||
| Conclusion: | As expected, significant and massive recruitment of neutrophils was observed in the lungs of infected mice compared to naive mice. neutrophils were found at similar counts in both sexes. No significant difference was observed between naive and infected mice as well as between male and female mice regarding the proportion of eosinophils | ||||
| Comments: | None | ||||
| Funding: | This research was supported by “IRIS research” | ||||
| Quality control: | Single color controls of each fluorochrome were run for compensation and FMO controls were run for gating | ||||
Experiment variables
| Conditions | |
|---|---|
| · Males GBS | 1. GBS M.fcs · 11_ male 24h.fcs · 19_ GBS M 24h.fcs · 20_ GBS M 24h.fcs · 22_ GBS M 24h.fcs · 23_ GBS M 24h.fcs · 25_ GBS M 24h.fcs · 26_ GBS M 24h.fcs |
| · Females GBS | 2. GBS F.fcs · 21_ GBS F 24h.fcs · 3 GBS F.fcs · 4. GBS F.fcs · 6_ GBS F 24h.fcs · 7_ GBS F 24h.fcs · 9_ GBS F 24h.fcs |
| · Males naives | 11_ PBS M 24h.fcs · 12_ PBS M 24h.fcs · 13_ PBS M 24h.fcs · 15_ PBS M 24h.fcs · 16_ PBS M 24h.fcs · 17_ PBS M 24h.fcs · 18_ PBS M 24h.fcs · 2_ Naive male.fcs |
| · Females naives | 14_ PBS F 24h.fcs · 1_ Naive Female.fcs · 1_ PBS F 24h.fcs · 2_ PBS F 24h.fcs · 3_ PBS F 24h.fcs · 4_ PBS F 24h.fcs · 5_ PBS F 24h.fcs · 5_ naive female.fcs |
