Experiment Overview
| Repository ID: | FR-FCM-Z25Y | Experiment name: | POC: T-cell kinetic responses following immunization with influenza antigens. | MIFlowCyt score: | 100.00% |
| Primary researcher: | Joost Vaneman | PI/manager: | Joost Vaneman | Uploaded by: | Joost Vaneman |
| Experiment dates: | 2015-02-19 - 2015-03-27 | Dataset uploaded: | Jun 2019 | Last updated: | Jul 2019 |
| Keywords: | [T cell] [kinetics] [Germinal center] | Manuscripts: | |||
| Organizations: |
Janssen Vaccines & Prevention B.V., Viral Vaccines, Leiden, (The Netherlands)
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| Purpose: | This experiment was performed to investigate weather frequencies of germinal center associated T cells,such as follicular T helper (TFH) cells (CD4+CXCR5+ Foxp3- PD1+ ICOS+ Bcl6+) and regulatory TFH's (TFR's: CD4+ CXCR5+ FoxP3+ PD1+), in the iliac lymph nodes of immunized mice could be associated with the protective efficacy of the used immunization regimen. | ||||
| Conclusion: | Unfortunately the analysis did not yield significant differences in TFH's and TFR's between the immunized cohorts (with the exception of the PBS mock contol). | ||||
| Comments: | we compared T cell subset frequencies between cohorts of mice subjected to immunization regimens inducing different levels of protection against lethal H1N1 influenza challenge. Naïve 8-weeks old mice were immunized three times, at days 0, 21 and 42 with different immunogens. One cohort of animals was vaccinated received a largely protective vaccine containing a high dose (30 µg) of a trimeric full-length H1 HA antigen (FL H1#2316) that provides heterologous protection against H1N1 A/Netherlands/602/2009 while a second cohort received a minimally protective vaccine containing a high dose (30 µg) of an HA stem-based monomeric antigen (UFV#4157) derived from FL H1#2316 To control for differences in immune responses related to the dose rather than intrinsically protective properties of the used immunization antigen, a lower but partiallly protective protective dose (0.3 µg) of FL H1#2316 was used for immunizations of a third cohort, while as a negative control for survival a fourth cohort was immunized with PBS only. All immunizations, including mock-immunizations with PBS, were adjuvanted with alum. To analyze cellular responses at early and late timepoints after prime or boost immunizations, 8 animals per cohort were sacrificed at 8 different timepoint (days 4, 7, 12, 19, 25, 28, 46 and 49). To assess the protective efficacy of the administered vaccine regimens, 10 animals per immunized cohort were challenged intranasally with a lethal dose of 25xLD50 H1N1 A/Netherlands/602/2009, heterologous to the parental H1 virus strain. After influenza challenge we observed that high (30 µg) and low (0.3 µg) doses of FL H1#2316 conferred survival rates of 80% and 60% respectively, while a high dose (30 µg) of UFV#4157 failed to provide any protection. All the animals that received PBS immunization succumbed to the infection. | ||||
| Funding: | N/A | ||||
| Quality control: | Before the experimental samples and compensation controls were acquired on the LSR Fortessa, a CST check was performed to verify the cytometers performance. CST Check Passed. | ||||
Experiment variables
| Doses | |
|---|---|
| · PBS | LN PBS_A4_A04_025.fcs · LN PBS_B4_B04_026.fcs · LN PBS_C4_C04_027.fcs · LN PBS_D4_D04_028.fcs · LN PBS_E4_E04_029.fcs · LN PBS_F4_F04_030.fcs · LN PBS_G4_G04_031.fcs · LN PBS_H4_H04_032.fcs · LN PBS_A4_A04_036.fcs · LN PBS_B4_B04_037.fcs · LN PBS_C4_C04_038.fcs · LN PBS_D4_D04_039.fcs · LN PBS_E4_E04_040.fcs · LN PBS_F4_F04_041.fcs · LN PBS_G4_G04_042.fcs · LN PBS_H4_H04_043.fcs · LN PBS_A4_A04_025.fcs-(1) · LN PBS_B4_B04_026.fcs-(1) · LN PBS_C4_C04_027.fcs-(1) · LN PBS_D4_D04_028.fcs-(1) · LN PBS_E4_E04_029.fcs-(1) · LN PBS_F4_F04_030.fcs-(1) · LN PBS_G4_G04_031.fcs-(1) · LN PBS_H4_H04_032.fcs-(1) · LN PBS_A4_A04_036.fcs-(1) · LN PBS_B4_B04_037.fcs-(1) · LN PBS_C4_C04_038.fcs-(1) · LN PBS_D4_D04_039.fcs-(1) · LN PBS_E4_E04_040.fcs-(1) · LN PBS_F4_F04_041.fcs-(1) · LN PBS_G4_G04_042.fcs-(1) · LN PBS_H4_H04_043.fcs-(1) · LN_PBS_A4_A04_025.fcs · LN_PBS_B4_B04_026.fcs · LN_PBS_C4_C04_027.fcs · LN_PBS_D4_D04_028.fcs · LN_PBS_E4_E04_029.fcs · LN_PBS_F4_F04_030.fcs · LN_PBS_G4_G04_031.fcs · LN_PBS_H4_H04_032.fcs · LN_PBS_A4_A04_025.fcs-(1) · LN_PBS_B4_B04_026.fcs-(1) · LN_PBS_C4_C04_027.fcs-(1) · LN_PBS_D4_D04_028.fcs-(1) · LN_PBS_E4_E04_029.fcs-(1) · LN_PBS_F4_F04_030.fcs-(1) · LN_PBS_G4_G04_031.fcs-(1) · LN_PBS_H4_H04_032.fcs-(1) · LN_PBS_A4_A04_036.fcs · LN_PBS_B4_B04_037.fcs · LN_PBS_C4_C04_038.fcs · LN_PBS_D4_D04_039.fcs · LN_PBS_E4_E04_040.fcs · LN_PBS_F4_F04_041.fcs · LN_PBS_G4_G04_042.fcs · LN_PBS_H4_H04_043.fcs · LN PBS_A4_A04_025.fcs-(2) · LN PBS_B4_B04_026.fcs-(2) · LN PBS_C4_C04_027.fcs-(2) · LN PBS_D4_D04_028.fcs-(2) · LN PBS_E4_E04_029.fcs-(2) · LN PBS_F4_F04_030.fcs-(2) · LN PBS_G4_G04_031.fcs-(2) · LN PBS_H4_H04_032.fcs-(2) |
| · 30µg FL H1#2316 | LN High dose FL HA_A1_A01_001.fcs · LN High dose FL HA_B1_B01_002.fcs · LN High dose FL HA_C1_C01_003.fcs · LN High dose FL HA_D1_D01_004.fcs · LN High dose FL HA_E1_E01_005.fcs · LN High dose FL HA_F1_F01_006.fcs · LN High dose FL HA_G1_G01_007.fcs · LN High dose FL HA_H1_H01_008.fcs · LN High Dose FL HA_A1_A01_012.fcs · LN High Dose FL HA_B1_B01_013.fcs · LN High Dose FL HA_C1_C01_014.fcs · LN High Dose FL HA_D1_D01_015.fcs · LN High Dose FL HA_E1_E01_016.fcs · LN High Dose FL HA_F1_F01_017.fcs · LN High Dose FL HA_G1_G01_018.fcs · LN High Dose FL HA_H1_H01_019.fcs · LN High dose FL HA_A1_A01_001.fcs-(1) · LN High dose FL HA_B1_B01_002.fcs-(1) · LN High dose FL HA_C1_C01_003.fcs-(1) · LN High dose FL HA_D1_D01_004.fcs-(1) · LN High dose FL HA_E1_E01_005.fcs-(1) · LN High dose FL HA_F1_F01_006.fcs-(1) · LN High dose FL HA_G1_G01_007.fcs-(1) · LN High dose FL HA_H1_H01_008.fcs-(1) · LN High Dose FL HA_A1_A01_012.fcs-(1) · LN High Dose FL HA_B1_B01_013.fcs-(1) · LN High Dose FL HA_C1_C01_014.fcs-(1) · LN High Dose FL HA_D1_D01_015.fcs-(1) · LN High Dose FL HA_E1_E01_016.fcs-(1) · LN High Dose FL HA_F1_F01_017.fcs-(1) · LN High Dose FL HA_G1_G01_018.fcs-(1) · LN High Dose FL HA_H1_H01_019.fcs-(1) · LN_HA_high_dose_A1_A01_001.fcs · LN_HA_high_dose_B1_B01_002.fcs · LN_HA_high_dose_C1_C01_003.fcs · LN_HA_high_dose_D1_D01_004.fcs · LN_HA_high_dose_E1_E01_005.fcs · LN_HA_high_dose_F1_F01_006.fcs · LN_HA_high_dose_G1_G01_007.fcs · LN_HA_high_dose_H1_H01_008.fcs · LN_high_dose_HA_A1_A01_001.fcs · LN_high_dose_HA_B1_B01_002.fcs · LN_high_dose_HA_C1_C01_003.fcs · LN_high_dose_HA_D1_D01_004.fcs · LN_high_dose_HA_E1_E01_005.fcs · LN_high_dose_HA_F1_F01_006.fcs · LN_high_dose_HA_G1_G01_007.fcs · LN_high_dose_HA_H1_H01_008.fcs · LN_High_Dose_FL_HA_A1_A01_012.fcs · LN_High_Dose_FL_HA_B1_B01_013.fcs · LN_High_Dose_FL_HA_C1_C01_014.fcs · LN_High_Dose_FL_HA_D1_D01_015.fcs · LN_High_Dose_FL_HA_E1_E01_016.fcs · LN_High_Dose_FL_HA_F1_F01_017.fcs · LN_High_Dose_FL_HA_G1_G01_018.fcs · LN_High_Dose_FL_HA_H1_H01_019.fcs · LN HA high_A1_A01_001.fcs · LN HA high_B1_B01_002.fcs · LN HA high_C1_C01_003.fcs · LN HA high_D1_D01_004.fcs · LN HA high_E1_E01_005.fcs · LN HA high_F1_F01_006.fcs · LN HA high_G1_G01_007.fcs · LN HA high_H1_H01_008.fcs |
| · 0.3µg FL H1#2316 | LN low dose FL HA_A2_A02_009.fcs · LN low dose FL HA_B2_B02_010.fcs · LN low dose FL HA_C2_C02_011.fcs · LN low dose FL HA_D2_D02_012.fcs · LN low dose FL HA_E2_E02_013.fcs · LN low dose FL HA_F2_F02_014.fcs · LN low dose FL HA_G2_G02_015.fcs · LN low dose FL HA_H2_H02_016.fcs · LN Low Dose FL HA_A2_A02_020.fcs · LN Low Dose FL HA_B2_B02_021.fcs · LN Low Dose FL HA_C2_C02_022.fcs · LN Low Dose FL HA_D2_D02_023.fcs · LN Low Dose FL HA_E2_E02_024.fcs · LN Low Dose FL HA_F2_F02_025.fcs · LN Low Dose FL HA_G2_G02_026.fcs · LN Low Dose FL HA_H2_H02_027.fcs · LN low dose FL HA_A2_A02_009.fcs-(1) · LN low dose FL HA_B2_B02_010.fcs-(1) · LN low dose FL HA_C2_C02_011.fcs-(1) · LN low dose FL HA_D2_D02_012.fcs-(1) · LN low dose FL HA_E2_E02_013.fcs-(1) · LN low dose FL HA_F2_F02_014.fcs-(1) · LN low dose FL HA_G2_G02_015.fcs-(1) · LN low dose FL HA_H2_H02_016.fcs-(1) · LN Low Dose FL HA_A2_A02_020.fcs-(1) · LN Low Dose FL HA_B2_B02_021.fcs-(1) · LN Low Dose FL HA_C2_C02_022.fcs-(1) · LN Low Dose FL HA_D2_D02_023.fcs-(1) · LN Low Dose FL HA_E2_E02_024.fcs-(1) · LN Low Dose FL HA_F2_F02_025.fcs-(1) · LN Low Dose FL HA_G2_G02_026.fcs-(1) · LN Low Dose FL HA_H2_H02_027.fcs-(1) · LN_HA_low_dose_A2_A02_009.fcs · LN_HA_low_dose_B2_B02_010.fcs · LN_HA_low_dose_C2_C02_011.fcs · LN_HA_low_dose_D2_D02_012.fcs · LN_HA_low_dose_E2_E02_013.fcs · LN_HA_low_dose_F2_F02_014.fcs · LN_HA_low_dose_G2_G02_015.fcs · LN_HA_low_dose_H2_H02_016.fcs · LN_low_dose_HA_A2_A02_009.fcs · LN_low_dose_HA_B2_B02_010.fcs · LN_low_dose_HA_C2_C02_011.fcs · LN_low_dose_HA_D2_D02_012.fcs · LN_low_dose_HA_E2_E02_013.fcs · LN_low_dose_HA_F2_F02_014.fcs · LN_low_dose_HA_G2_G02_015.fcs · LN_low_dose_HA_H2_H02_016.fcs · LN_low_Dose_FL_HA_A2_A02_020.fcs · LN_low_Dose_FL_HA_B2_B02_021.fcs · LN_low_Dose_FL_HA_C2_C02_022.fcs · LN_low_Dose_FL_HA_D2_D02_023.fcs · LN_low_Dose_FL_HA_E2_E02_024.fcs · LN_low_Dose_FL_HA_F2_F02_025.fcs · LN_low_Dose_FL_HA_G2_G02_026.fcs · LN_low_Dose_FL_HA_H2_H02_027.fcs · LN HA low_A2_A02_009.fcs · LN HA low_B2_B02_010.fcs · LN HA low_C2_C02_011.fcs · LN HA low_D2_D02_012.fcs · LN HA low_E2_E02_013.fcs · LN HA low_F2_F02_014.fcs · LN HA low_G2_G02_015.fcs · LN HA low_H2_H02_016.fcs |
| · 30µg UFV#4157 | LN High dose mini HA_A3_A03_017.fcs · LN High dose mini HA_B3_B03_018.fcs · LN High dose mini HA_C3_C03_019.fcs · LN High dose mini HA_D3_D03_020.fcs · LN High dose mini HA_E3_E03_021.fcs · LN High dose mini HA_F3_F03_022.fcs · LN High dose mini HA_G3_G03_023.fcs · LN High dose mini HA_H3_H03_024.fcs · LN High Dose miniHA_A3_A03_028.fcs · LN High Dose miniHA_B3_B03_029.fcs · LN High Dose miniHA_C3_C03_030.fcs · LN High Dose miniHA_D3_D03_031.fcs · LN High Dose miniHA_E3_E03_032.fcs · LN High Dose miniHA_F3_F03_033.fcs · LN High Dose miniHA_G3_G03_034.fcs · LN High Dose miniHA_H3_H03_035.fcs · LN High dose miniHA_A3_A03_017.fcs · LN High dose miniHA_B3_B03_018.fcs · LN High dose miniHA_C3_C03_019.fcs · LN High dose miniHA_D3_D03_020.fcs · LN High dose miniHA_E3_E03_021.fcs · LN High dose miniHA_F3_F03_022.fcs · LN High dose miniHA_G3_G03_023.fcs · LN High dose miniHA_H3_H03_024.fcs · LN High Dose miniHA_A3_A03_028.fcs-(1) · LN High Dose miniHA_B3_B03_029.fcs-(1) · LN High Dose miniHA_C3_C03_030.fcs-(1) · LN High Dose miniHA_D3_D03_031.fcs-(1) · LN High Dose miniHA_E3_E03_032.fcs-(1) · LN High Dose miniHA_F3_F03_033.fcs-(1) · LN High Dose miniHA_G3_G03_034.fcs-(1) · LN High Dose miniHA_H3_H03_035.fcs-(1) · LN_miniHA_high_dose_A3_A03_017.fcs · LN_miniHA_high_dose_B3_B03_018.fcs · LN_miniHA_high_dose_C3_C03_019.fcs · LN_miniHA_high_dose_D3_D03_020.fcs · LN_miniHA_high_dose_E3_E03_021.fcs · LN_miniHA_high_dose_F3_F03_022.fcs · LN_miniHA_high_dose_G3_G03_023.fcs · LN_miniHA_high_dose_H3_H03_024.fcs · LN_high_dose_miniHA_A3_A03_017.fcs · LN_high_dose_miniHA_B3_B03_018.fcs · LN_high_dose_miniHA_C3_C03_019.fcs · LN_high_dose_miniHA_D3_D03_020.fcs · LN_high_dose_miniHA_E3_E03_021.fcs · LN_high_dose_miniHA_F3_F03_022.fcs · LN_high_dose_miniHA_G3_G03_023.fcs · LN_high_dose_miniHA_H3_H03_024.fcs · LN_High_Dose_miniHA_A3_A03_028.fcs · LN_High_Dose_miniHA_B3_B03_029.fcs · LN_High_Dose_miniHA_C3_C03_030.fcs · LN_High_Dose_miniHA_D3_D03_031.fcs · LN_High_Dose_miniHA_E3_E03_032.fcs · LN_High_Dose_miniHA_F3_F03_033.fcs · LN_High_Dose_miniHA_G3_G03_034.fcs · LN_High_Dose_miniHA_H3_H03_035.fcs · LN miniHA high_A3_A03_017.fcs · LN miniHA high_B3_B03_018.fcs · LN miniHA high_C3_C03_019.fcs · LN miniHA high_D3_D03_020.fcs · LN miniHA high_E3_E03_021.fcs · LN miniHA high_F3_F03_022.fcs · LN miniHA high_G3_G03_023.fcs · LN miniHA high_H3_H03_024.fcs |
| Sample Type | |
|---|---|
| · iliac lymph nodes | LN High dose FL HA_A1_A01_001.fcs · LN High dose FL HA_B1_B01_002.fcs · LN High dose FL HA_C1_C01_003.fcs · LN High dose FL HA_D1_D01_004.fcs · LN High dose FL HA_E1_E01_005.fcs · LN High dose FL HA_F1_F01_006.fcs · LN High dose FL HA_G1_G01_007.fcs · LN High dose FL HA_H1_H01_008.fcs · LN High dose mini HA_A3_A03_017.fcs · LN High dose mini HA_B3_B03_018.fcs · LN High dose mini HA_C3_C03_019.fcs · LN High dose mini HA_D3_D03_020.fcs · LN High dose mini HA_E3_E03_021.fcs · LN High dose mini HA_F3_F03_022.fcs · LN High dose mini HA_G3_G03_023.fcs · LN High dose mini HA_H3_H03_024.fcs · LN PBS_A4_A04_025.fcs · LN PBS_B4_B04_026.fcs · LN PBS_C4_C04_027.fcs · LN PBS_D4_D04_028.fcs · LN PBS_E4_E04_029.fcs · LN PBS_F4_F04_030.fcs · LN PBS_G4_G04_031.fcs · LN PBS_H4_H04_032.fcs · LN low dose FL HA_A2_A02_009.fcs · LN low dose FL HA_B2_B02_010.fcs · LN low dose FL HA_C2_C02_011.fcs · LN low dose FL HA_D2_D02_012.fcs · LN low dose FL HA_E2_E02_013.fcs · LN low dose FL HA_F2_F02_014.fcs · LN low dose FL HA_G2_G02_015.fcs · LN low dose FL HA_H2_H02_016.fcs · LN High Dose FL HA_A1_A01_012.fcs · LN High Dose FL HA_B1_B01_013.fcs · LN High Dose FL HA_C1_C01_014.fcs · LN High Dose FL HA_D1_D01_015.fcs · LN High Dose FL HA_E1_E01_016.fcs · LN High Dose FL HA_F1_F01_017.fcs · LN High Dose FL HA_G1_G01_018.fcs · LN High Dose FL HA_H1_H01_019.fcs · LN High Dose miniHA_A3_A03_028.fcs · LN High Dose miniHA_B3_B03_029.fcs · LN High Dose miniHA_C3_C03_030.fcs · LN High Dose miniHA_D3_D03_031.fcs · LN High Dose miniHA_E3_E03_032.fcs · LN High Dose miniHA_F3_F03_033.fcs · LN High Dose miniHA_G3_G03_034.fcs · LN High Dose miniHA_H3_H03_035.fcs · LN Low Dose FL HA_A2_A02_020.fcs · LN Low Dose FL HA_B2_B02_021.fcs · LN Low Dose FL HA_C2_C02_022.fcs · LN Low Dose FL HA_D2_D02_023.fcs · LN Low Dose FL HA_E2_E02_024.fcs · LN Low Dose FL HA_F2_F02_025.fcs · LN Low Dose FL HA_G2_G02_026.fcs · LN Low Dose FL HA_H2_H02_027.fcs · LN PBS_A4_A04_036.fcs · LN PBS_B4_B04_037.fcs · LN PBS_C4_C04_038.fcs · LN PBS_D4_D04_039.fcs · LN PBS_E4_E04_040.fcs · LN PBS_F4_F04_041.fcs · LN PBS_G4_G04_042.fcs · LN PBS_H4_H04_043.fcs · LN High dose FL HA_A1_A01_001.fcs-(1) · LN High dose FL HA_B1_B01_002.fcs-(1) · LN High dose FL HA_C1_C01_003.fcs-(1) · LN High dose FL HA_D1_D01_004.fcs-(1) · LN High dose FL HA_E1_E01_005.fcs-(1) · LN High dose FL HA_F1_F01_006.fcs-(1) · LN High dose FL HA_G1_G01_007.fcs-(1) · LN High dose FL HA_H1_H01_008.fcs-(1) · LN High dose miniHA_A3_A03_017.fcs · LN High dose miniHA_B3_B03_018.fcs · LN High dose miniHA_C3_C03_019.fcs · LN High dose miniHA_D3_D03_020.fcs · LN High dose miniHA_E3_E03_021.fcs · LN High dose miniHA_F3_F03_022.fcs · LN High dose miniHA_G3_G03_023.fcs · LN High dose miniHA_H3_H03_024.fcs · LN PBS_A4_A04_025.fcs-(1) · LN PBS_B4_B04_026.fcs-(1) · LN PBS_C4_C04_027.fcs-(1) · LN PBS_D4_D04_028.fcs-(1) · LN PBS_E4_E04_029.fcs-(1) · LN PBS_F4_F04_030.fcs-(1) · LN PBS_G4_G04_031.fcs-(1) · LN PBS_H4_H04_032.fcs-(1) · LN low dose FL HA_A2_A02_009.fcs-(1) · LN low dose FL HA_B2_B02_010.fcs-(1) · LN low dose FL HA_C2_C02_011.fcs-(1) · LN low dose FL HA_D2_D02_012.fcs-(1) · LN low dose FL HA_E2_E02_013.fcs-(1) · LN low dose FL HA_F2_F02_014.fcs-(1) · LN low dose FL HA_G2_G02_015.fcs-(1) · LN low dose FL HA_H2_H02_016.fcs-(1) · LN High Dose FL HA_A1_A01_012.fcs-(1) · LN High Dose FL HA_B1_B01_013.fcs-(1) · LN High Dose FL HA_C1_C01_014.fcs-(1) · LN High Dose FL HA_D1_D01_015.fcs-(1) · LN High Dose FL HA_E1_E01_016.fcs-(1) · LN High Dose FL HA_F1_F01_017.fcs-(1) · LN High Dose FL HA_G1_G01_018.fcs-(1) · LN High Dose FL HA_H1_H01_019.fcs-(1) · LN High Dose miniHA_A3_A03_028.fcs-(1) · LN High Dose miniHA_B3_B03_029.fcs-(1) · LN High Dose miniHA_C3_C03_030.fcs-(1) · LN High Dose miniHA_D3_D03_031.fcs-(1) · LN High Dose miniHA_E3_E03_032.fcs-(1) · LN High Dose miniHA_F3_F03_033.fcs-(1) · LN High Dose miniHA_G3_G03_034.fcs-(1) · LN High Dose miniHA_H3_H03_035.fcs-(1) · LN Low Dose FL HA_A2_A02_020.fcs-(1) · LN Low Dose FL HA_B2_B02_021.fcs-(1) · LN Low Dose FL HA_C2_C02_022.fcs-(1) · LN Low Dose FL HA_D2_D02_023.fcs-(1) · LN Low Dose FL HA_E2_E02_024.fcs-(1) · LN Low Dose FL HA_F2_F02_025.fcs-(1) · LN Low Dose FL HA_G2_G02_026.fcs-(1) · LN Low Dose FL HA_H2_H02_027.fcs-(1) · LN PBS_A4_A04_036.fcs-(1) · LN PBS_B4_B04_037.fcs-(1) · LN PBS_C4_C04_038.fcs-(1) · LN PBS_D4_D04_039.fcs-(1) · LN PBS_E4_E04_040.fcs-(1) · LN PBS_F4_F04_041.fcs-(1) · LN PBS_G4_G04_042.fcs-(1) · LN PBS_H4_H04_043.fcs-(1) · LN_HA_high_dose_A1_A01_001.fcs · LN_HA_high_dose_B1_B01_002.fcs · LN_HA_high_dose_C1_C01_003.fcs · LN_HA_high_dose_D1_D01_004.fcs · LN_HA_high_dose_E1_E01_005.fcs · LN_HA_high_dose_F1_F01_006.fcs · LN_HA_high_dose_G1_G01_007.fcs · LN_HA_high_dose_H1_H01_008.fcs · LN_HA_low_dose_A2_A02_009.fcs · LN_HA_low_dose_B2_B02_010.fcs · LN_HA_low_dose_C2_C02_011.fcs · LN_HA_low_dose_D2_D02_012.fcs · LN_HA_low_dose_E2_E02_013.fcs · LN_HA_low_dose_F2_F02_014.fcs · LN_HA_low_dose_G2_G02_015.fcs · LN_HA_low_dose_H2_H02_016.fcs · LN_PBS_A4_A04_025.fcs · LN_PBS_B4_B04_026.fcs · LN_PBS_C4_C04_027.fcs · LN_PBS_D4_D04_028.fcs · LN_PBS_E4_E04_029.fcs · LN_PBS_F4_F04_030.fcs · LN_PBS_G4_G04_031.fcs · LN_PBS_H4_H04_032.fcs · LN_miniHA_high_dose_A3_A03_017.fcs · LN_miniHA_high_dose_B3_B03_018.fcs · LN_miniHA_high_dose_C3_C03_019.fcs · LN_miniHA_high_dose_D3_D03_020.fcs · LN_miniHA_high_dose_E3_E03_021.fcs · LN_miniHA_high_dose_F3_F03_022.fcs · LN_miniHA_high_dose_G3_G03_023.fcs · LN_miniHA_high_dose_H3_H03_024.fcs · LN_PBS_A4_A04_025.fcs-(1) · LN_PBS_B4_B04_026.fcs-(1) · LN_PBS_C4_C04_027.fcs-(1) · LN_PBS_D4_D04_028.fcs-(1) · LN_PBS_E4_E04_029.fcs-(1) · LN_PBS_F4_F04_030.fcs-(1) · LN_PBS_G4_G04_031.fcs-(1) · LN_PBS_H4_H04_032.fcs-(1) · LN_high_dose_HA_A1_A01_001.fcs · LN_high_dose_HA_B1_B01_002.fcs · LN_high_dose_HA_C1_C01_003.fcs · LN_high_dose_HA_D1_D01_004.fcs · LN_high_dose_HA_E1_E01_005.fcs · LN_high_dose_HA_F1_F01_006.fcs · LN_high_dose_HA_G1_G01_007.fcs · LN_high_dose_HA_H1_H01_008.fcs · LN_high_dose_miniHA_A3_A03_017.fcs · LN_high_dose_miniHA_B3_B03_018.fcs · LN_high_dose_miniHA_C3_C03_019.fcs · LN_high_dose_miniHA_D3_D03_020.fcs · LN_high_dose_miniHA_E3_E03_021.fcs · LN_high_dose_miniHA_F3_F03_022.fcs · LN_high_dose_miniHA_G3_G03_023.fcs · LN_high_dose_miniHA_H3_H03_024.fcs · LN_low_dose_HA_A2_A02_009.fcs · LN_low_dose_HA_B2_B02_010.fcs · LN_low_dose_HA_C2_C02_011.fcs · LN_low_dose_HA_D2_D02_012.fcs · LN_low_dose_HA_E2_E02_013.fcs · LN_low_dose_HA_F2_F02_014.fcs · LN_low_dose_HA_G2_G02_015.fcs · LN_low_dose_HA_H2_H02_016.fcs · LN_High_Dose_FL_HA_A1_A01_012.fcs · LN_High_Dose_FL_HA_B1_B01_013.fcs · LN_High_Dose_FL_HA_C1_C01_014.fcs · LN_High_Dose_FL_HA_D1_D01_015.fcs · LN_High_Dose_FL_HA_E1_E01_016.fcs · LN_High_Dose_FL_HA_F1_F01_017.fcs · LN_High_Dose_FL_HA_G1_G01_018.fcs · LN_High_Dose_FL_HA_H1_H01_019.fcs · LN_High_Dose_miniHA_A3_A03_028.fcs · LN_High_Dose_miniHA_B3_B03_029.fcs · LN_High_Dose_miniHA_C3_C03_030.fcs · LN_High_Dose_miniHA_D3_D03_031.fcs · LN_High_Dose_miniHA_E3_E03_032.fcs · LN_High_Dose_miniHA_F3_F03_033.fcs · LN_High_Dose_miniHA_G3_G03_034.fcs · LN_High_Dose_miniHA_H3_H03_035.fcs · LN_PBS_A4_A04_036.fcs · LN_PBS_B4_B04_037.fcs · LN_PBS_C4_C04_038.fcs · LN_PBS_D4_D04_039.fcs · LN_PBS_E4_E04_040.fcs · LN_PBS_F4_F04_041.fcs · LN_PBS_G4_G04_042.fcs · LN_PBS_H4_H04_043.fcs · LN_low_Dose_FL_HA_A2_A02_020.fcs · LN_low_Dose_FL_HA_B2_B02_021.fcs · LN_low_Dose_FL_HA_C2_C02_022.fcs · LN_low_Dose_FL_HA_D2_D02_023.fcs · LN_low_Dose_FL_HA_E2_E02_024.fcs · LN_low_Dose_FL_HA_F2_F02_025.fcs · LN_low_Dose_FL_HA_G2_G02_026.fcs · LN_low_Dose_FL_HA_H2_H02_027.fcs · LN HA high_A1_A01_001.fcs · LN HA high_B1_B01_002.fcs · LN HA high_C1_C01_003.fcs · LN HA high_D1_D01_004.fcs · LN HA high_E1_E01_005.fcs · LN HA high_F1_F01_006.fcs · LN HA high_G1_G01_007.fcs · LN HA high_H1_H01_008.fcs · LN HA low_A2_A02_009.fcs · LN HA low_B2_B02_010.fcs · LN HA low_C2_C02_011.fcs · LN HA low_D2_D02_012.fcs · LN HA low_E2_E02_013.fcs · LN HA low_F2_F02_014.fcs · LN HA low_G2_G02_015.fcs · LN HA low_H2_H02_016.fcs · LN PBS_A4_A04_025.fcs-(2) · LN PBS_B4_B04_026.fcs-(2) · LN PBS_C4_C04_027.fcs-(2) · LN PBS_D4_D04_028.fcs-(2) · LN PBS_E4_E04_029.fcs-(2) · LN PBS_F4_F04_030.fcs-(2) · LN PBS_G4_G04_031.fcs-(2) · LN PBS_H4_H04_032.fcs-(2) · LN miniHA high_A3_A03_017.fcs · LN miniHA high_B3_B03_018.fcs · LN miniHA high_C3_C03_019.fcs · LN miniHA high_D3_D03_020.fcs · LN miniHA high_E3_E03_021.fcs · LN miniHA high_F3_F03_022.fcs · LN miniHA high_G3_G03_023.fcs · LN miniHA high_H3_H03_024.fcs |
| Timepoints | |
|---|---|
| · Day 4 | LN High dose FL HA_A1_A01_001.fcs · LN High dose FL HA_B1_B01_002.fcs · LN High dose FL HA_C1_C01_003.fcs · LN High dose FL HA_D1_D01_004.fcs · LN High dose FL HA_E1_E01_005.fcs · LN High dose FL HA_F1_F01_006.fcs · LN High dose FL HA_G1_G01_007.fcs · LN High dose FL HA_H1_H01_008.fcs · LN High dose mini HA_A3_A03_017.fcs · LN High dose mini HA_B3_B03_018.fcs · LN High dose mini HA_C3_C03_019.fcs · LN High dose mini HA_D3_D03_020.fcs · LN High dose mini HA_E3_E03_021.fcs · LN High dose mini HA_F3_F03_022.fcs · LN High dose mini HA_G3_G03_023.fcs · LN High dose mini HA_H3_H03_024.fcs · LN PBS_A4_A04_025.fcs · LN PBS_B4_B04_026.fcs · LN PBS_C4_C04_027.fcs · LN PBS_D4_D04_028.fcs · LN PBS_E4_E04_029.fcs · LN PBS_F4_F04_030.fcs · LN PBS_G4_G04_031.fcs · LN PBS_H4_H04_032.fcs · LN low dose FL HA_A2_A02_009.fcs · LN low dose FL HA_B2_B02_010.fcs · LN low dose FL HA_C2_C02_011.fcs · LN low dose FL HA_D2_D02_012.fcs · LN low dose FL HA_E2_E02_013.fcs · LN low dose FL HA_F2_F02_014.fcs · LN low dose FL HA_G2_G02_015.fcs · LN low dose FL HA_H2_H02_016.fcs |
| · Day 7 | LN High Dose FL HA_A1_A01_012.fcs · LN High Dose FL HA_B1_B01_013.fcs · LN High Dose FL HA_C1_C01_014.fcs · LN High Dose FL HA_D1_D01_015.fcs · LN High Dose FL HA_E1_E01_016.fcs · LN High Dose FL HA_F1_F01_017.fcs · LN High Dose FL HA_G1_G01_018.fcs · LN High Dose FL HA_H1_H01_019.fcs · LN High Dose miniHA_A3_A03_028.fcs · LN High Dose miniHA_B3_B03_029.fcs · LN High Dose miniHA_C3_C03_030.fcs · LN High Dose miniHA_D3_D03_031.fcs · LN High Dose miniHA_E3_E03_032.fcs · LN High Dose miniHA_F3_F03_033.fcs · LN High Dose miniHA_G3_G03_034.fcs · LN High Dose miniHA_H3_H03_035.fcs · LN Low Dose FL HA_A2_A02_020.fcs · LN Low Dose FL HA_B2_B02_021.fcs · LN Low Dose FL HA_C2_C02_022.fcs · LN Low Dose FL HA_D2_D02_023.fcs · LN Low Dose FL HA_E2_E02_024.fcs · LN Low Dose FL HA_F2_F02_025.fcs · LN Low Dose FL HA_G2_G02_026.fcs · LN Low Dose FL HA_H2_H02_027.fcs · LN PBS_A4_A04_036.fcs · LN PBS_B4_B04_037.fcs · LN PBS_C4_C04_038.fcs · LN PBS_D4_D04_039.fcs · LN PBS_E4_E04_040.fcs · LN PBS_F4_F04_041.fcs · LN PBS_G4_G04_042.fcs · LN PBS_H4_H04_043.fcs |
| · Day 12 | LN High dose FL HA_A1_A01_001.fcs-(1) · LN High dose FL HA_B1_B01_002.fcs-(1) · LN High dose FL HA_C1_C01_003.fcs-(1) · LN High dose FL HA_D1_D01_004.fcs-(1) · LN High dose FL HA_E1_E01_005.fcs-(1) · LN High dose FL HA_F1_F01_006.fcs-(1) · LN High dose FL HA_G1_G01_007.fcs-(1) · LN High dose FL HA_H1_H01_008.fcs-(1) · LN High dose miniHA_A3_A03_017.fcs · LN High dose miniHA_B3_B03_018.fcs · LN High dose miniHA_C3_C03_019.fcs · LN High dose miniHA_D3_D03_020.fcs · LN High dose miniHA_E3_E03_021.fcs · LN High dose miniHA_F3_F03_022.fcs · LN High dose miniHA_G3_G03_023.fcs · LN High dose miniHA_H3_H03_024.fcs · LN PBS_A4_A04_025.fcs-(1) · LN PBS_B4_B04_026.fcs-(1) · LN PBS_C4_C04_027.fcs-(1) · LN PBS_D4_D04_028.fcs-(1) · LN PBS_E4_E04_029.fcs-(1) · LN PBS_F4_F04_030.fcs-(1) · LN PBS_G4_G04_031.fcs-(1) · LN PBS_H4_H04_032.fcs-(1) · LN low dose FL HA_A2_A02_009.fcs-(1) · LN low dose FL HA_B2_B02_010.fcs-(1) · LN low dose FL HA_C2_C02_011.fcs-(1) · LN low dose FL HA_D2_D02_012.fcs-(1) · LN low dose FL HA_E2_E02_013.fcs-(1) · LN low dose FL HA_F2_F02_014.fcs-(1) · LN low dose FL HA_G2_G02_015.fcs-(1) · LN low dose FL HA_H2_H02_016.fcs-(1) |
| · Day 19 | LN High Dose FL HA_A1_A01_012.fcs-(1) · LN High Dose FL HA_B1_B01_013.fcs-(1) · LN High Dose FL HA_C1_C01_014.fcs-(1) · LN High Dose FL HA_D1_D01_015.fcs-(1) · LN High Dose FL HA_E1_E01_016.fcs-(1) · LN High Dose FL HA_F1_F01_017.fcs-(1) · LN High Dose FL HA_G1_G01_018.fcs-(1) · LN High Dose FL HA_H1_H01_019.fcs-(1) · LN High Dose miniHA_A3_A03_028.fcs-(1) · LN High Dose miniHA_B3_B03_029.fcs-(1) · LN High Dose miniHA_C3_C03_030.fcs-(1) · LN High Dose miniHA_D3_D03_031.fcs-(1) · LN High Dose miniHA_E3_E03_032.fcs-(1) · LN High Dose miniHA_F3_F03_033.fcs-(1) · LN High Dose miniHA_G3_G03_034.fcs-(1) · LN High Dose miniHA_H3_H03_035.fcs-(1) · LN Low Dose FL HA_A2_A02_020.fcs-(1) · LN Low Dose FL HA_B2_B02_021.fcs-(1) · LN Low Dose FL HA_C2_C02_022.fcs-(1) · LN Low Dose FL HA_D2_D02_023.fcs-(1) · LN Low Dose FL HA_E2_E02_024.fcs-(1) · LN Low Dose FL HA_F2_F02_025.fcs-(1) · LN Low Dose FL HA_G2_G02_026.fcs-(1) · LN Low Dose FL HA_H2_H02_027.fcs-(1) · LN PBS_A4_A04_036.fcs-(1) · LN PBS_B4_B04_037.fcs-(1) · LN PBS_C4_C04_038.fcs-(1) · LN PBS_D4_D04_039.fcs-(1) · LN PBS_E4_E04_040.fcs-(1) · LN PBS_F4_F04_041.fcs-(1) · LN PBS_G4_G04_042.fcs-(1) · LN PBS_H4_H04_043.fcs-(1) |
| · Day 25 | LN_HA_high_dose_A1_A01_001.fcs · LN_HA_high_dose_B1_B01_002.fcs · LN_HA_high_dose_C1_C01_003.fcs · LN_HA_high_dose_D1_D01_004.fcs · LN_HA_high_dose_E1_E01_005.fcs · LN_HA_high_dose_F1_F01_006.fcs · LN_HA_high_dose_G1_G01_007.fcs · LN_HA_high_dose_H1_H01_008.fcs · LN_HA_low_dose_A2_A02_009.fcs · LN_HA_low_dose_B2_B02_010.fcs · LN_HA_low_dose_C2_C02_011.fcs · LN_HA_low_dose_D2_D02_012.fcs · LN_HA_low_dose_E2_E02_013.fcs · LN_HA_low_dose_F2_F02_014.fcs · LN_HA_low_dose_G2_G02_015.fcs · LN_HA_low_dose_H2_H02_016.fcs · LN_PBS_A4_A04_025.fcs · LN_PBS_B4_B04_026.fcs · LN_PBS_C4_C04_027.fcs · LN_PBS_D4_D04_028.fcs · LN_PBS_E4_E04_029.fcs · LN_PBS_F4_F04_030.fcs · LN_PBS_G4_G04_031.fcs · LN_PBS_H4_H04_032.fcs · LN_miniHA_high_dose_A3_A03_017.fcs · LN_miniHA_high_dose_B3_B03_018.fcs · LN_miniHA_high_dose_C3_C03_019.fcs · LN_miniHA_high_dose_D3_D03_020.fcs · LN_miniHA_high_dose_E3_E03_021.fcs · LN_miniHA_high_dose_F3_F03_022.fcs · LN_miniHA_high_dose_G3_G03_023.fcs · LN_miniHA_high_dose_H3_H03_024.fcs |
| · Day 28 | LN_PBS_A4_A04_025.fcs-(1) · LN_PBS_B4_B04_026.fcs-(1) · LN_PBS_C4_C04_027.fcs-(1) · LN_PBS_D4_D04_028.fcs-(1) · LN_PBS_E4_E04_029.fcs-(1) · LN_PBS_F4_F04_030.fcs-(1) · LN_PBS_G4_G04_031.fcs-(1) · LN_PBS_H4_H04_032.fcs-(1) · LN_high_dose_HA_A1_A01_001.fcs · LN_high_dose_HA_B1_B01_002.fcs · LN_high_dose_HA_C1_C01_003.fcs · LN_high_dose_HA_D1_D01_004.fcs · LN_high_dose_HA_E1_E01_005.fcs · LN_high_dose_HA_F1_F01_006.fcs · LN_high_dose_HA_G1_G01_007.fcs · LN_high_dose_HA_H1_H01_008.fcs · LN_high_dose_miniHA_A3_A03_017.fcs · LN_high_dose_miniHA_B3_B03_018.fcs · LN_high_dose_miniHA_C3_C03_019.fcs · LN_high_dose_miniHA_D3_D03_020.fcs · LN_high_dose_miniHA_E3_E03_021.fcs · LN_high_dose_miniHA_F3_F03_022.fcs · LN_high_dose_miniHA_G3_G03_023.fcs · LN_high_dose_miniHA_H3_H03_024.fcs · LN_low_dose_HA_A2_A02_009.fcs · LN_low_dose_HA_B2_B02_010.fcs · LN_low_dose_HA_C2_C02_011.fcs · LN_low_dose_HA_D2_D02_012.fcs · LN_low_dose_HA_E2_E02_013.fcs · LN_low_dose_HA_F2_F02_014.fcs · LN_low_dose_HA_G2_G02_015.fcs · LN_low_dose_HA_H2_H02_016.fcs |
| · Day 46 | LN_High_Dose_FL_HA_A1_A01_012.fcs · LN_High_Dose_FL_HA_B1_B01_013.fcs · LN_High_Dose_FL_HA_C1_C01_014.fcs · LN_High_Dose_FL_HA_D1_D01_015.fcs · LN_High_Dose_FL_HA_E1_E01_016.fcs · LN_High_Dose_FL_HA_F1_F01_017.fcs · LN_High_Dose_FL_HA_G1_G01_018.fcs · LN_High_Dose_FL_HA_H1_H01_019.fcs · LN_High_Dose_miniHA_A3_A03_028.fcs · LN_High_Dose_miniHA_B3_B03_029.fcs · LN_High_Dose_miniHA_C3_C03_030.fcs · LN_High_Dose_miniHA_D3_D03_031.fcs · LN_High_Dose_miniHA_E3_E03_032.fcs · LN_High_Dose_miniHA_F3_F03_033.fcs · LN_High_Dose_miniHA_G3_G03_034.fcs · LN_High_Dose_miniHA_H3_H03_035.fcs · LN_PBS_A4_A04_036.fcs · LN_PBS_B4_B04_037.fcs · LN_PBS_C4_C04_038.fcs · LN_PBS_D4_D04_039.fcs · LN_PBS_E4_E04_040.fcs · LN_PBS_F4_F04_041.fcs · LN_PBS_G4_G04_042.fcs · LN_PBS_H4_H04_043.fcs · LN_low_Dose_FL_HA_A2_A02_020.fcs · LN_low_Dose_FL_HA_B2_B02_021.fcs · LN_low_Dose_FL_HA_C2_C02_022.fcs · LN_low_Dose_FL_HA_D2_D02_023.fcs · LN_low_Dose_FL_HA_E2_E02_024.fcs · LN_low_Dose_FL_HA_F2_F02_025.fcs · LN_low_Dose_FL_HA_G2_G02_026.fcs · LN_low_Dose_FL_HA_H2_H02_027.fcs |
| · Day 49 | LN HA high_A1_A01_001.fcs · LN HA high_B1_B01_002.fcs · LN HA high_C1_C01_003.fcs · LN HA high_D1_D01_004.fcs · LN HA high_E1_E01_005.fcs · LN HA high_F1_F01_006.fcs · LN HA high_G1_G01_007.fcs · LN HA high_H1_H01_008.fcs · LN HA low_A2_A02_009.fcs · LN HA low_B2_B02_010.fcs · LN HA low_C2_C02_011.fcs · LN HA low_D2_D02_012.fcs · LN HA low_E2_E02_013.fcs · LN HA low_F2_F02_014.fcs · LN HA low_G2_G02_015.fcs · LN HA low_H2_H02_016.fcs · LN PBS_A4_A04_025.fcs-(2) · LN PBS_B4_B04_026.fcs-(2) · LN PBS_C4_C04_027.fcs-(2) · LN PBS_D4_D04_028.fcs-(2) · LN PBS_E4_E04_029.fcs-(2) · LN PBS_F4_F04_030.fcs-(2) · LN PBS_G4_G04_031.fcs-(2) · LN PBS_H4_H04_032.fcs-(2) · LN miniHA high_A3_A03_017.fcs · LN miniHA high_B3_B03_018.fcs · LN miniHA high_C3_C03_019.fcs · LN miniHA high_D3_D03_020.fcs · LN miniHA high_E3_E03_021.fcs · LN miniHA high_F3_F03_022.fcs · LN miniHA high_G3_G03_023.fcs · LN miniHA high_H3_H03_024.fcs |
