Experiment Overview

Repository ID: FR-FCM-Z5X5 Experiment name: 32 marker mass cytometry panel for T cells MIFlowCyt score: 49.25%
Primary researcher: Alexis Sponaugle PI/manager: Alexis Sponaugle Uploaded by: Alexis Sponaugle
Experiment dates: 2021-08-10 - 2022-01-20 Dataset uploaded: Aug 2022 Last updated: Sep 2022
Keywords: [mass cytometry] [CyTOF] [CD4 T cells; CD8 T cells] Manuscripts:
Organizations: University of North Carolina at Chapel Hill, Chapel Hill, NC (USA)
Purpose: To standardize a 32 marker mass cytometry panel able to characterize CD4 and CD8 T cells.
Conclusion: This panel is able to identify all canonical lineage markers as well as CD4 T cell helper subsets, CD4 and CD8 T cell activation and memory subsets
Comments: None
Funding: Not disclosed
Quality control: The same human PBMCs were spiked into all fully stained samples tagged with CD45 111Cd in order to confirm optimal staining and account for batch effects.
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