Experiment Overview
| Repository ID: | FR-FCM-Z7WX | Experiment name: | Expression analysis of cTnT and IRX4 in human heart-on-a-chip | MIFlowCyt score: | 59.79% |
| Primary researcher: | Ken Takahashi | PI/manager: | Ken Takahashi | Uploaded by: | Ken Takahashi |
| Experiment dates: | 2022-04-01 - 2024-03-31 | Dataset uploaded: | May 2024 | Last updated: | May 2024 |
| Keywords: | [Endothelial cells] [fIbroblast] [human iPSCs] [Cardiomyocytes] | Manuscripts: | |||
| Organizations: |
Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, (Japan)
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| Purpose: | Compare the expression of cardiac marker proteins, cardiac troponin T (TNNT2), and IRX4 between the biculture (fibroblast and hiPS-cardiomyocyte) and triculture (endothelial cell, fibroblast, and hiPS-cardiomyocyte) in the heart-on-a-chip. | ||||
| Conclusion: | The tri-culture group exhibited significantly higher IRX4 expression compared to the bi-culture group. Additionally, the percentage of cells co-expressing cTnT and IRX4 was notably elevated in the tri-culture group in contrast to the bi-culture group. | ||||
| Comments: | None | ||||
| Funding: | This research received support from the Japan Society for the Promotion of Science (JSPS) through Grant-in-Aid for Scientific Research (B) (No. 20H04518). | ||||
| Quality control: | FCS collection for software testing | Using control beads | |||
Experiment variables
| Conditions | |
|---|---|
| · control | iPS-control.fcs |
| · biculture | ID22072804-Biculture.fcs · ID22072805-Biculture.fcs · ID22072806-Biculture.fcs · ID23042404-Biculture.fcs · ID23042405-Biculture.fcs · ID23042406-Biculture.fcs |
| · triculture | ID22072801-Triculture.fcs · ID22072802-Triculture.fcs · ID22072803-Triculture.fcs · ID23042403-Triculture.fcs · ID23052301-Triculture.fcs |
