Experiment Overview
| Repository ID: | FR-FCM-Z8EP | Experiment name: | Viable Cell Barcoding with SeMal and TeMal | MIFlowCyt score: | 51.50% |
| Primary researcher: | Marie Iannone | PI/manager: | Marie Iannone | Uploaded by: | Marie Iannone |
| Experiment dates: | 2023-03-01 - 2023-03-31 | Dataset uploaded: | Oct 2024 | Last updated: | Oct 2024 |
| Keywords: | [mass cytometry] [CyTOF] [SeMal] [TeMal] [selenium maleimide] [tellurium maleimide] [mass-tag cell barcoding] | Manuscripts: | [39582135] |
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| Organizations: |
University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina (USA)
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| Purpose: | Pooled Antibody Staining of Viable PBMC after SeMal/TeMal 21-plex Barcoding. Twenty-one individual PBMC samples (PBMC from seven separate donors with 3 replicates per donor) were barcoded using the SeMal/TeMal isotope scheme shown in Figure 5A and Supplemental Figure S5A of publication 24-120.R1 Cytometry Part A. The barcoded samples were mixed, viability stained then incubated, as a pool, with a pre-titrated 33-marker antibody cocktail (See Supplemental Table S1 for list of markers and metals). After surface marker staining, cells were fixed with 1.6% paraformaldehyde and incubated with iridium overnight. | ||||
| Conclusion: | Each donor sample replicate retained its unique phenotypic profile with excellent reproducibility. | ||||
| Comments: | Data used in Figure 6 of publication 24-120.R1 Cytometry Part A. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | This is a bead normalized fcs file. | ||||
