Experiment Overview
| Repository ID: | FR-FCM-Z74A | Experiment name: | RNAi-based Boolean gates in the yeast S. cerevisiae | MIFlowCyt score: | 47.00% |
| Primary researcher: | Ximing Tian | PI/manager: | Mario Andrea Marchisio | Uploaded by: | Ximing Tian |
| Experiment dates: | 2021-10-01 - 2023-12-01 | Dataset uploaded: | Dec 2023 | Last updated: | Dec 2023 |
| Keywords: | [siRNA] [Boolean gates] | Manuscripts: | |||
| Organizations: |
Tianjin University, School of Pharmaceutical Science and Technology, Tianjin, (China)
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| Purpose: | The efficiency of the circuits is evaluated by measuring the expression levels of green fluorescence. | ||||
| Conclusion: | We successfully constructed circuits that can produce dsRNA molecular structures and evaluated the functionality of these circuits. | ||||
| Comments: | All samples were measured at least three independent times at different time. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
Experiment variables
| Individuals | |
|---|---|
| · byMM584-negative control | byMM584-16h-1.fcs · byMM584-16h-2.fcs · byMM584-16h-3.fcs · byMM584-16h-4.fcs · byMM584-16h-5.fcs · byMM584-24h-1.fcs · byMM584-24h-2.fcs · byMM584-24h-3.fcs · byMM584-24h-4.fcs · byMM584-24h-5.fcs |
| · Figure 2 | byMM1696-GAL-1.fcs · byMM1696-GAL-2.fcs · byMM1696-GAL-3.fcs · byMM1696-GLU-1.fcs · byMM1696-GLU-2.fcs · byMM1696-GLU-3.fcs · byMM1699-GAL-1.fcs · byMM1699-GAL-2.fcs · byMM1699-GAL-3.fcs · byMM1699-GLU-1.fcs · byMM1699-GLU-2.fcs · byMM1699-GLU-3.fcs · byMM1846-COPPER-1.fcs · byMM1846-COPPER-2.fcs · byMM1846-COPPER-3.fcs · byMM1846-COPPER-4.fcs · byMM1846-GLU-1.fcs · byMM1846-GLU-2.fcs · byMM1846-GLU-3.fcs · byMM1864-COPPER-1.fcs · byMM1864-COPPER-2.fcs · byMM1864-COPPER-3.fcs · byMM1864-COPPER-4.fcs · byMM1864-COPPER-5.fcs · byMM1864-GLU-1.fcs · byMM1864-GLU-2.fcs · byMM1864-GLU-3.fcs · byMM1864-GLU-4.fcs · byMM1864-GLU-5.fcs |
| · Figure 3 | byMM1900 SDC+Me+GAL-1.fcs · byMM1900 SDC+Me+GAL-2.fcs · byMM1900 SDC+Me+GAL-3.fcs · byMM1900 SDC+Me-1.fcs · byMM1900 SDC+Me-2.fcs · byMM1900 SDC+Me-3.fcs · byMM1900 SDC+Me-4.fcs · byMM1900 SDC+Me-5.fcs · byMM1900 SDC-Me+GAL-1.fcs · byMM1900 SDC-Me+GAL-2.fcs · byMM1900 SDC-Me+GAL-3.fcs · byMM1900 SDC-Me-1.fcs · byMM1900 SDC-Me-2.fcs · byMM1900 SDC-Me-3.fcs |
| · Figure 4 | byMM103-GLU-1.fcs · byMM103-GLU-2.fcs · byMM103-GLU-3.fcs · byMM224-GAL-1.fcs · byMM224-GAL-2.fcs · byMM224-GAL-3.fcs · byMM224-GLU-1.fcs · byMM224-GLU-2.fcs · byMM224-GLU-3.fcs · byMM282-GLU-1.fcs · byMM282-GLU-2.fcs · byMM282-GLU-3.fcs · byMM282-GLU-4.fcs · byMM282-GLU-5.fcs · byMM292-GAL-1.fcs · byMM292-GAL-2.fcs · byMM292-GAL-3.fcs · byMM292-GAL-4.fcs · byMM292-GLU-1.fcs · byMM292-GLU-2.fcs · byMM292-GLU-3.fcs |
| · Figure 5 | byMM276-GLU-1.fcs · byMM276-GLU-2.fcs · byMM276-GLU-3.fcs · byMM277-GLU-1.fcs · byMM277-GLU-2.fcs · byMM277-GLU-3.fcs · byMM283-GLU-1.fcs · byMM283-GLU-2.fcs · byMM283-GLU-3.fcs · byMM283-GLU-4.fcs · byMM283-GLU-5.fcs · byMM283-GLU-6.fcs · byMM283-GLU-7.fcs · byMM307-GLU-1.fcs · byMM307-GLU-2.fcs · byMM307-GLU-3.fcs · byMM308-GLU-1.fcs · byMM308-GLU-2.fcs · byMM308-GLU-3.fcs · byMM308-GLU-4.fcs · byMM312-GLU-1.fcs · byMM312-GLU-2.fcs · byMM312-GLU-3.fcs · byMM313-GLU-1.fcs · byMM313-GLU-2.fcs · byMM313-GLU-3.fcs · byMM313-GLU-4.fcs · byMM313-GLU-5.fcs |
| · Figure 6 | byMM283-GAL-1.fcs · byMM283-GAL-2.fcs · byMM283-GAL-3.fcs · byMM283-GAL-4.fcs · byMM305-GAL-1.fcs · byMM305-GAL-2.fcs · byMM305-GAL-3.fcs · byMM305-GLU-1.fcs · byMM305-GLU-2.fcs · byMM305-GLU-3.fcs · byMM306-GAL-1.fcs · byMM306-GAL-2.fcs · byMM306-GAL-3.fcs · byMM306-GAL-4.fcs · byMM306-GLU-1.fcs · byMM306-GLU-2.fcs · byMM306-GLU-3.fcs · byMM333-GAL-1.fcs · byMM333-GAL-2.fcs · byMM333-GAL-3.fcs · byMM333-GLU-1.fcs · byMM333-GLU-2.fcs · byMM333-GLU-3.fcs · byMM333-GLU-4.fcs · byMM334-GAL-1.fcs · byMM334-GAL-2.fcs · byMM334-GAL-3.fcs · byMM334-GLU-1.fcs · byMM334-GLU-2.fcs · byMM334-GLU-3.fcs |
| · Figure 7 | byMM1912 GAL-1.fcs · byMM1912 GAL-2.fcs · byMM1912 GAL-3.fcs · byMM1912 GLU-1.fcs · byMM1912 GLU-2.fcs · byMM1912 GLU-3.fcs · byMM1912 GLU-4.fcs · byMM1912 SDC+Me-1.fcs · byMM1912 SDC+Me-2.fcs · byMM1912 SDC+Me-3.fcs · byMM1912 SDC+Me-4.fcs · byMM1912 SDC-Me-1.fcs · byMM1912 SDC-Me-2.fcs · byMM1912 SDC-Me-3.fcs · byMM1912 SDC-Me-4.fcs · byMM1917 GAL-1.fcs · byMM1917 GAL-2.fcs · byMM1917 GAL-3.fcs · byMM1917 GLU-1.fcs · byMM1917 GLU-2.fcs · byMM1917 GLU-3.fcs · byMM1917 GLU-4.fcs · byMM1917 GLU-5.fcs · byMM1964 SDC+Me-1.fcs · byMM1964 SDC+Me-2.fcs · byMM1964 SDC+Me-3.fcs · byMM1964 SDC+Me-4.fcs · byMM1964 SDC-Me-1.fcs · byMM1964 SDC-Me-2.fcs · byMM1964 SDC-Me-3.fcs |
| · Figure 8 | byMM1918 SDC+Me+GAL-1.fcs · byMM1918 SDC+Me+GAL-2.fcs · byMM1918 SDC+Me+GAL-3.fcs · byMM1918 SDC+Me+GAL-4.fcs · byMM1918 SDC+Me-1.fcs · byMM1918 SDC+Me-2.fcs · byMM1918 SDC+Me-3.fcs · byMM1918 SDC+Me-4.fcs · byMM1918 SDC+Me-5.fcs · byMM1918 SDC-ME-2.fcs · byMM1918 SDC-Me+GAL-1.fcs · byMM1918 SDC-Me+GAL-2.fcs · byMM1918 SDC-Me+GAL-3.fcs · byMM1918 SDC-Me-1.fcs · byMM1918 SDC-Me-3.fcs |
| · Figure 9 | byMM2018-GAL-1.fcs · byMM2018-GAL-2.fcs · byMM2018-GAL-3.fcs · byMM2018-GLU-1.fcs · byMM2018-GLU-2.fcs · byMM2018-GLU-3.fcs · byMM348-GAL-1.fcs · byMM348-GAL-2.fcs · byMM348-GAL-3.fcs · byMM348-GLU-1.fcs · byMM348-GLU-2.fcs · byMM348-GLU-3.fcs · byMM348-GLU-4.fcs · byMM352-GAL-1.fcs · byMM352-GAL-2.fcs · byMM352-GAL-3.fcs · byMM352-GLU-1.fcs · byMM352-GLU-2.fcs · byMM352-GLU-3.fcs · byMM352-GLU-4.fcs |
| · Figure S1 | byMM1849 GAL-1.fcs · byMM1849 GAL-2.fcs · byMM1849 GAL-3.fcs · byMM1849 GLU-1.fcs · byMM1849 GLU-2.fcs · byMM1849 GLU-3.fcs · byMM1852 GAL-1.fcs · byMM1852 GAL-2.fcs · byMM1852 GAL-3.fcs · byMM1852 GLU-1.fcs · byMM1852 GLU-2.fcs · byMM1852 GLU-3.fcs · byMM380 GAL-1.fcs · byMM380 GAL-2.fcs · byMM380 GAL-3.fcs · byMM380 GLU-1.fcs · byMM380 GLU-2.fcs · byMM380 GLU-3.fcs |
| · Figure S2 | byMM355 SDC-URA+GAL-1.fcs · byMM355 SDC-URA+GAL-2.fcs · byMM355 SDC-URA+GAL-3.fcs · byMM355 SDC-URA-1.fcs · byMM355 SDC-URA-2.fcs · byMM355 SDC-URA-3.fcs · byMM356 SDC-URA+GAL-1.fcs · byMM356 SDC-URA+GAL-2.fcs · byMM356 SDC-URA+GAL-3.fcs · byMM356 SDC-URA-1.fcs · byMM356 SDC-URA-2.fcs · byMM356 SDC-URA-3.fcs |
| · Figure S3 | byMM1849 GAL-3.fcs-(1) · byMM1849 GLU-2.fcs-(1) · byMM1851 GAL-1.fcs · byMM1851 GAL-2.fcs · byMM1851 GAL-3.fcs · byMM1851 GAL-4.fcs · byMM1851 GLU-1.fcs · byMM1851 GLU-2.fcs · byMM1851 GLU-3.fcs · byMM1854 GAL-1.fcs · byMM1854 GAL-2.fcs · byMM1854 GAL-3.fcs · byMM1854 GAL-4.fcs · byMM1854 GAL-5.fcs · byMM1854 GAL-6.fcs · byMM1854 GLU-1.fcs · byMM1854 GLU-2.fcs · byMM1854 GLU-3.fcs |
| · Figure S5 | byMM1897 SDC+Me-1.fcs · byMM1897 SDC+Me-2.fcs · byMM1897 SDC+Me-3.fcs · byMM1897 SDC-Me-1.fcs · byMM1897 SDC-Me-2.fcs · byMM1897 SDC-Me-3.fcs · byMM1914 SDC+Me-1.fcs · byMM1914 SDC+Me-2.fcs · byMM1914 SDC+Me-3.fcs · byMM1914 SDC-Me-1.fcs · byMM1914 SDC-Me-2.fcs · byMM1914 SDC-Me-3.fcs |
| · Figure S6 | byMM1976 SDC+Me+COPPER-1.fcs · byMM1976 SDC+Me+COPPER-2.fcs · byMM1976 SDC+Me+COPPER-3.fcs · byMM1976 SDC+Me-COPPER-1.fcs · byMM1976 SDC+Me-COPPER-2.fcs · byMM1976 SDC+Me-COPPER-3.fcs · byMM1976 SDC-Me+COPPER-1.fcs · byMM1976 SDC-Me+COPPER-2.fcs · byMM1976 SDC-Me+COPPER-3.fcs · byMM1976 SDC-Me-COPPER-1.fcs · byMM1976 SDC-Me-COPPER-2.fcs · byMM1976 SDC-Me-COPPER-3.fcs |
| · Figure S7 | byMM1912 SDC+COPPER-1.fcs · byMM1912 SDC+COPPER-2.fcs · byMM1912 SDC+COPPER-3.fcs · byMM1912 SDC-COPPER-2.fcs · byMM1912 SDC-COPPER-3.fcs · byMM1975 SDC+COPPER-1.fcs · byMM1975 SDC+COPPER-2.fcs · byMM1975 SDC+COPPER-3.fcs · byMM1975 SDC-COPPER-1.fcs · byMM1975 SDC-COPPER-2.fcs · byMM1975 SDC-COPPER-3.fcs |
