Experiment Overview
| Repository ID: | FR-FCM-Z4ST | Experiment name: | imaging mass cytometry for breast cancer tissue | MIFlowCyt score: | 27.00% |
| Primary researcher: | Zijian Feng | PI/manager: | Zijian Feng | Uploaded by: | Zijian Feng |
| Experiment dates: | 2020-12-01 - 2021-01-31 | Dataset uploaded: | Jan 2022 | Last updated: | Jan 2022 |
| Keywords: | [Bacteria] [LPS] [B cell] [Breast Cancer] [immune cell] [CD8+T cell] [LTA] | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | Breast cancer tissue section samples from five patients from each of the four types of breast cancer (TNBC, HER2+, Luminal A, Luminal B) were stained to analyze bacteria, immune cells, cancer cells, CD8+ T cells, and B cells. | ||||
| Conclusion: | The designed panel performed well in the follow-up analysis, where LPS was detected in all four breast cancer samples and can be used for the detection of Gram+ bacteria in tumor microecology. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Applying MCD Viewer to the raw data for image output, then CellProfiler was used for cell segmentation, and finally histoCAT was applied for cell clustering and neighborhood analysis. | ||||
