Experiment Overview
| Repository ID: | FR-FCM-Z46J | Experiment name: | gp120 VLPs | MIFlowCyt score: | 63.25% |
| Primary researcher: | John Nolan | PI/manager: | John Nolan | Uploaded by: | John Nolan |
| Experiment dates: | 2020-07-01 - 2021-06-30 | Dataset uploaded: | Jul 2021 | Last updated: | Jul 2021 |
| Keywords: | [extracellular vesicle] [VLP] [gp120] [Gag] [vesicle flow cytometry.] | Manuscripts: | [34679128] [PMC8565784] |
|
|
| Organizations: |
Scintillon Institute, San Diego, CA (USA)
|
||||
| Purpose: | To measure the expression of antigens on virus like particles (VLPs) | ||||
| Conclusion: | Co-expression of Gag and gp120 increases the total amount of gp120-bearing VLPs produced, but the fraction positive for gp120 | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Instrument performance was characterized using a combination of multi-intensity single fluorophore beads (Quantum FITC, Bangs Labs Quantibrite PE, BD Biosciences) whose intensity had been calibrated in units of MESF, multi-intensity multifluorophore beads (vCal nanoRainbow, Cellarcus), and antibody capture beads (vCal antibody capture beads, Cellarcus) calibrated to report results in units of antibodies bound per vesicle (ABV). EV analysis by vesicle flow cytometry (VFC) was conducted and reported as suggested by the MIFlowCyt-EV guidelines (See attached checklist). | ||||
