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Experiment Overview

Repository ID: FR-FCM-Z3XB Experiment name: Novel S. cerevisiae hybrid synthetic promoters based on foreign core promoter sequences MIFlowCyt score: 41.00%
Primary researcher: FENG Xiaofan PI/manager: Mario Andrea Marchisio Uploaded by: Feng Xiaofan
Experiment dates: 2020-06-18 - 2021-03-12 Dataset uploaded: Apr 2021 Last updated: Apr 2021
Keywords: None Manuscripts:
Organizations: Tianjin University, School of Pharmaceutical Science and Technology, Tianjin, (China)
Purpose: In this work we constructed and optimized new synthetic constitutive promoters for the yeast Saccharomyces cerevisiae. We start from foreign (e.g. viral) core promoters as templates. They are, usually, unfunctional in yeast but can be activated by extending them with a short sequence, from the CYC1 promoter, containing various transcription start sites (TSSs). Overall, we extended the pool of S. cerevisiae promoters with 59 new samples, the strongest overcoming the native TEF2 promoter.
Conclusion: None
Comments: None
Funding: Not disclosed
Quality control: None


Experiment variables

Individuals
· Figure 2 103-1.fcs · 103-2.fcs · 103-3.fcs · 131-1.fcs · 131-2.fcs · 131-3.fcs · 134-1.fcs · 134-2.fcs · 134-3.fcs · 14-1.fcs · 14-2.fcs · 14-3.fcs · 143-1.fcs · 143-2.fcs · 143-3.fcs · 20-1.fcs · 20-2.fcs · 20-3.fcs · 205-1.fcs · 205-2.fcs · 205-3.fcs · 331-1.fcs · 38-1.fcs · 38-2.fcs · 38-3.fcs · 57-1.fcs · 57-2.fcs · 57-3.fcs · 783-1.fcs · 783-2.fcs · 783-3.fcs · 869-1.fcs · 869-2.fcs · 869-3.fcs · 871-1.fcs · 871-2.fcs · 871-3.fcs · 872-1.fcs · 872-2.fcs · 872-3.fcs · 873-1.fcs · 873-2.fcs · 873-3.fcs · 874-1.fcs · 874-2.fcs · 874-3.fcs · 876-1.fcs · 876-2.fcs · 876-3.fcs · 89-1.fcs · 89-2.fcs · 89-3.fcs · 920-1.fcs · 920-2.fcs · 920-3.fcs · 921-1.fcs · 921-2.fcs · 921-3.fcs · by331-2.fcs
· Figure 3 893-1.fcs · 893-2.fcs · 893-3.fcs · 901-1.fcs · 901-2.fcs · 901-3.fcs · 902-1.fcs · 902-2.fcs · 902-3.fcs · 903-1.fcs · 903-2.fcs · 903-3.fcs · 904-1.fcs · 904-2.fcs · 904-3.fcs · 905-1.fcs · 905-2.fcs · 905-3.fcs · 906-1.fcs · 906-2.fcs · 906-3.fcs · 907-1.fcs · 907-2.fcs · 907-3.fcs · 916-1.fcs · 916-2.fcs · 916-3.fcs · 918-1.fcs · 918-2.fcs · 918-3.fcs · 919-1.fcs · 919-2.fcs · 919-3.fcs · 940-1.fcs · 940-2.fcs · 940-3.fcs · 941-1.fcs · 941-2.fcs · 941-3.fcs · 942-1.fcs · 942-2.fcs · 942-3.fcs · 945-1.fcs · 945-2.fcs · 945-3.fcs · 970-1.fcs · 970-2.fcs · 970-3.fcs · 971-1.fcs · 971-2.fcs · 971-3.fcs · 972-1.fcs · 972-2.fcs · 972-3.fcs · 973-1.fcs · 973-2.fcs · 973-3.fcs · 974-1.fcs · 974-2.fcs · 974-3.fcs · 975-1.fcs · 975-2.fcs · 975-3.fcs · 976-1.fcs · 976-2.fcs · 976-3.fcs · 977-1.fcs · 977-2.fcs · 977-3.fcs · 978-1.fcs · 978-2.fcs · 978-3.fcs · 979-1.fcs · 979-2.fcs · 979-3.fcs · 980-1.fcs · 980-2.fcs · 980-3.fcs
· Figure 4 1142-1.fcs · 1142-2.fcs · 1142-3.fcs · 1143-1.fcs · 1143-2.fcs · 1143-3.fcs · 1144-1.fcs · 1144-2.fcs · 1144-3.fcs · 947-1.fcs · 947-2.fcs · 947-3.fcs · 948-1.fcs · 948-2.fcs · 948-3.fcs · 949-1.fcs · 949-2.fcs · 949-3.fcs · 981-1.fcs · 981-2.fcs · 981-3.fcs · 982-1.fcs · 982-2.fcs · 982-3.fcs · 983-1.fcs · 983-2.fcs · 983-3.fcs
· Figure 5 1170-1.fcs · 1170-2.fcs · 1170-3.fcs · 1171-1.fcs · 1171-2.fcs · 1171-3.fcs · 1172-1.fcs · 1172-2.fcs · 1172-3.fcs · 1203-1.fcs · 1203-2.fcs · 1203-3.fcs · 1204-1.fcs · 1204-2.fcs · 1204-3.fcs · 1205-1.fcs · 1205-2.fcs · 1205-3.fcs · 1206-1.fcs · 1206-2.fcs · 1206-3.fcs · 1231-1.fcs · 1231-2.fcs · 1231-3.fcs
· Figure6 1230-1.fcs · 1230-2.fcs · 1230-3.fcs · 1254-1.fcs · 1254-2.fcs · 1254-3.fcs · 1255-1.fcs · 1255-2.fcs · 1255-3.fcs · 1256-1.fcs · 1256-2.fcs · 1256-3.fcs
· Supplementary figures 1232-1.fcs · 1232-2.fcs · 1232-3.fcs · 868-1.fcs · 868-2.fcs · 868-3.fcs · 877-1.fcs · 877-2.fcs · 877-3.fcs · 894-1.fcs · 894-2.fcs · 894-3.fcs · 900-1.fcs · 900-2.fcs · 900-3.fcs

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