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Experiment Overview

Repository ID: FR-FCM-Z5S2 Experiment name: Mass-cytometry for the multiplexed quantification and characterization of target expression on circulating cells in whole blood MIFlowCyt score: 49.31%
Primary researcher: Chad Stevens PI/manager: Chad Stevens Uploaded by: Chad Stevens
Experiment dates: 2014-12-04 - 2021-05-19 Dataset uploaded: Oct 2022 Last updated: Oct 2022
Keywords: [mass cytometry] [CyTOF] [receptor quantification] Manuscripts:
Organizations: Pfizer, Andover, Massachusetts (United States)
Purpose: This paper describes the method and case study for multiple receptor quantification of circulating whole blood cells using mass cytometry. CD4, CD8, CD28, CD16, CD64 and CD32a are quantified in whole blood with high parameter mass cytometry panels.
Conclusion: Shows mass cytometry can be utilized for multiple receptor quantification of targets in human whole blood
Comments: None
Funding: none
Quality control: used 0ug-mL samples as negative controls to gate on target antibodies


Experiment variables

Sample Type
· Whole Blood c01_WB_01_1_CAT_3.1ug-mL_Donor 1.fcs · c01_WB_01_1_CAT_3.1ug-mL_Donor 4.fcs · c01_WB_01_1_CAT_3.1ug-mL_Donor 5.fcs · c02_WB_01_1_CAT_0.8ug-mL_Donor 1.fcs · c02_WB_01_1_CAT_0.8ug-mL_Donor 4.fcs · c02_WB_01_1_CAT_0.8ug-mL_Donor 5.fcs · c03_WB_01_1_CAT_0.4ug-mL_Donor 1.fcs · c03_WB_01_1_CAT_0.4ug-mL_Donor 4.fcs · c03_WB_01_1_CAT_0.4ug-mL_Donor 5.fcs · c04_WB_01_1_CAT_0.2ug-mL_Donor 1.fcs · c04_WB_01_1_CAT_0.2ug-mL_Donor 4.fcs · c04_WB_01_1_CAT_0.2ug-mL_Donor 5.fcs · c05_WB_01_1_CAT_0.1ug-mL_Donor 1.fcs · c05_WB_01_1_CAT_0.1ug-mL_Donor 4.fcs · c05_WB_01_1_CAT_0.1ug-mL_Donor 5.fcs · c06_WB_01_1_CAT_0.05ug-mL_Donor 1.fcs · c06_WB_01_1_CAT_0.05ug-mL_Donor 4.fcs · c06_WB_01_1_CAT_0.05ug-mL_Donor 5.fcs · c07_WB_01_1_CAT_0.025ug-mL_Donor 1.fcs · c07_WB_01_1_CAT_0.025ug-mL_Donor 4.fcs · c07_WB_01_1_CAT_0.025ug-mL_Donor 5.fcs · c08_WB_01_1_CAT_0.012ug-mL_Donor 1.fcs · c08_WB_01_1_CAT_0.012ug-mL_Donor 4.fcs · c08_WB_01_1_CAT_0.012ug-mL_Donor 5.fcs · c09_WB_01_1_CAT_0,006ug-mL_Donor 1.fcs · c09_WB_01_1_CAT_0,006ug-mL_Donor 4.fcs · c09_WB_01_1_CAT_0,006ug-mL_Donor 5.fcs · c10_WB_01_1_CAT_0ug-mL_Donor 1.fcs · c10_WB_01_1_CAT_0ug-mL_Donor 4.fcs · c10_WB_01_1_CAT_0ug-mL_Donor 5.fcs · c11_WB_01_1_CAT_3.1ug-mL_Donor 2.fcs · c11_WB_01_1_CAT_3.1ug-mL_Donor 3.fcs · c11_WB_01_1_CAT_3.1ug-mL_Donor 6.fcs · c12_WB_01_1_CAT_0.8ug-mL_Donor 2.fcs · c12_WB_01_1_CAT_0.8ug-mL_Donor 3.fcs · c12_WB_01_1_CAT_0.8ug-mL_Donor 6.fcs · c13_WB_01_1_CAT_0.4ug-mL_Donor 2.fcs · c13_WB_01_1_CAT_0.4ug-mL_Donor 3.fcs · c13_WB_01_1_CAT_0.4ug-mL_Donor 6.fcs · c14_WB_01_1_CAT_0.2ug-mL_Donor 2.fcs · c14_WB_01_1_CAT_0.2ug-mL_Donor 3.fcs · c14_WB_01_1_CAT_0.2ug-mL_Donor 6.fcs · c15_WB_01_1_CAT_0.1ug-mL_Donor 2.fcs · c15_WB_01_1_CAT_0.1ug-mL_Donor 3.fcs · c15_WB_01_1_CAT_0.1ug-mL_Donor 6.fcs · c16_WB_01_1_CAT_0.05ug-mL_Donor 2.fcs · c16_WB_01_1_CAT_0.05ug-mL_Donor 3.fcs · c16_WB_01_1_CAT_0.05ug-mL_Donor 6.fcs · c17_WB_01_1_CAT_0.025ug-mL_Donor 2.fcs · c17_WB_01_1_CAT_0.025ug-mL_Donor 3.fcs · c17_WB_01_1_CAT_0.025ug-mL_Donor 6.fcs · c18_WB_01_1_CAT_0.012ug-mL_Donor 2.fcs · c18_WB_01_1_CAT_0.012ug-mL_Donor 3.fcs · c18_WB_01_1_CAT_0.012ug-mL_Donor 6.fcs · c19_WB_01_1_CAT_0,006ug-mL_Donor 2.fcs · c19_WB_01_1_CAT_0,006ug-mL_Donor 3.fcs · c19_WB_01_1_CAT_0,006ug-mL_Donor 6.fcs · c20_WB_01_1_CAT_0ug-mL_Donor 2.fcs · c20_WB_01_1_CAT_0ug-mL_Donor 3.fcs · c20_WB_01_1_CAT_0ug-mL_Donor 6.fcs

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