Experiment Overview
| Repository ID: | FR-FCM-Z7B8 | Experiment name: | Cell proliferation | MIFlowCyt score: | 52.25% |
| Primary researcher: | Drífa Guðmundsdóttir | PI/manager: | Stefan Sigurdsson | Uploaded by: | Drífa Guðmundsdóttir |
| Experiment dates: | 2022-04-07 - 2022-10-11 | Dataset uploaded: | Apr 2024 | Last updated: | Apr 2024 |
| Keywords: | [U2OS] [MITF] | Manuscripts: | |||
| Organizations: |
University of Iceland, Faculty of medicine, Reykjavik, (Iceland)
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| Purpose: | Analyzing cell proliferation of U2OS cells after siRNA mediated MITF knockdown. | ||||
| Conclusion: | MITF knockdown slows down U2OS cell proliferation. | ||||
| Comments: | None | ||||
| Funding: | Icelandic research fund and Icelandic cancer association research fund. | ||||
| Quality control: | CELL DEATH ANALYSIS | Daily performance test of instrument. Compensation not required for this assay. | |||
Experiment variables
| Timepoints | |
|---|---|
| · 0 | proliferation dhg(2)_Group_day 0.fcs · proliferation dhg_Group_day 0.fcs |
| · 5 days | proliferation dhg(2)_Group_siCON.fcs · proliferation dhg(2)_Group_siMITF.fcs · proliferation dhg(2)_Group_siMITF_P53.fcs · proliferation dhg(2)_Group_siP53.fcs · proliferation dhg_Group_siCON 2.fcs · proliferation dhg_Group_siMITF 2.fcs · proliferation dhg_Group_siMITF_P53.fcs · proliferation dhg_Group_siP53 2.fcs · proliferation dhg_Group_siCON.fcs · proliferation dhg_Group_siMITF.fcs · proliferation dhg_Group_siMITF_P.fcs · proliferation dhg_Group_siP53.fcs |
| Conditions | |
|---|---|
| · siCON | proliferation dhg(2)_Group_siCON.fcs · proliferation dhg_Group_siCON 2.fcs · proliferation dhg_Group_siCON.fcs |
| · siMITF | proliferation dhg(2)_Group_siMITF.fcs · proliferation dhg_Group_siMITF 2.fcs · proliferation dhg_Group_siMITF.fcs |
| · siP53 | proliferation dhg(2)_Group_siP53.fcs · proliferation dhg_Group_siP53 2.fcs · proliferation dhg_Group_siP53.fcs |
| · siMITF_siP53 | proliferation dhg(2)_Group_siMITF_P53.fcs · proliferation dhg_Group_siMITF_P53.fcs · proliferation dhg_Group_siMITF_P.fcs |
| · untreated | proliferation dhg(2)_Group_day 0.fcs · proliferation dhg_Group_day 0.fcs |
