Experiment Overview
| Repository ID: | FR-FCM-ZYUZ | Experiment name: | Antibody bound per neisserial cell experiment - Replicate 3 | MIFlowCyt score: | 46.25% |
| Primary researcher: | Ronni A G da Silva | PI/manager: | Ruth Griffin | Uploaded by: | Ronni A G da Silva |
| Experiment dates: | 2018-03-03 - 2018-05-05 | Dataset uploaded: | Dec 2018 | Last updated: | Dec 2018 |
| Keywords: | [FACS] [meningococcus] [fhbp] | Manuscripts: | |||
| Organizations: |
The University of Nottingham, Life Sciences, Nottingham, (UK)
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| Purpose: | Evaluate the level of cell surface exposure of FHbp on 20 different strains. Antibody Bound per cell was acquired based on a curve made with beads that have a known concentration antibody bound to them. | ||||
| Conclusion: | None | ||||
| Comments: | Meningococcal cells were gated and fluorescence detected against its respective negative control that was stained with secondary antibody only. | ||||
| Funding: | CNPq The University of Nottingham | ||||
| Quality control: | All samples were initially incubated with a monoclonal antibody agains FHbp protein (Jar4) and incubated with the secondary antibody rat anti IgG conjugated with FITC Strains with secondary antibody only only was used as control | ||||
