Experiment Overview
Repository ID: | FR-FCM-Z6UL | Experiment name: | Cell cycle synchronisation and analysis HeLa1.3 cells | MIFlowCyt score: | 72.75% |
Primary researcher: | Emilia Herrera Moyano | PI/manager: | Emilia Herrera Moyano | Uploaded by: | Emilia Herrera Moyano |
Experiment dates: | 2017-06-02 - | Dataset uploaded: | Oct 2023 | Last updated: | Nov 2023 |
Keywords: | [cell cycle] [EdU PI] [double thymidine block] [HeLa1.3 cells] | Manuscripts: | |||
Organizations: |
Institute of Clinical Sciences, Imperial College London, London, (UK)
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Purpose: | Cell cycle synchronization of HeLa1.3 using double thymidine block and release. Collection and FACS analysis each hour after the release. Cell cycle synchronization of HeLa1.3 using thymidine block, release and enrichment of mitotic population using nocodazole. | ||||
Conclusion: | None | ||||
Comments: | None | ||||
Funding: | Not disclosed | ||||
Quality control: | Single stained controls were included. Samples were stained and acquired in parallel. |