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Experiment: 10d protein expansion
ID: FR-FCM-Z582
Primary Researcher: Emily Tang
MIFlowCyt Score: 39.50%

Experiment Overview

Repository ID:	FR-FCM-Z582	Experiment name:	10d protein expansion	MIFlowCyt score:	39.50%
Primary researcher:	Emily Tang	PI/manager:	Emily Tang	Uploaded by:	Emily Tang
Experiment dates:	2020-10-01 - 2021-07-31	Dataset uploaded:	Apr 2022	Last updated:	Apr 2022
Keywords:	None		Manuscripts:	[35668177]
Organizations:	None
Purpose:	To determine whether there are CD4 and CD8 T cell responses recognising SpCas9, SaCas9 and RfxCas13d using recombinant proteins
Conclusion:	None
Comments:	None
Funding:	Not disclosed
Quality control:	Compensation controls were run during every acquisition session. Compensation values were exported directly in the fcs files and further verified during analysis using Flowjo.

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The following open access article describes how to upload and annotate flow cytometry data sets: Spidlen J, Breuer K and Brinkman R. Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (FlowRepository.org). Current Protocols in Cytometry, UNIT 10.18, July 2012.
We also have a Quick start guide and a FAQ section.
You may download slides from our Workshop at CYTO 2012: Publishing MIFlowCyt Compliant Data to ISAC’s FlowRepository.org for Cytometry A and Other Journals
Additional links and help options are listed in our support page.
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Citing FlowRepository

Please reference us by citing:

Spidlen J, Breuer K, Rosenberg C, Kotecha N and Brinkman RR. FlowRepository - A Resource of Annotated Flow Cytometry Datasets Associated with Peer-reviewed Publications. Cytometry A. 2012 Sep; 81(9):727-31..

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