Experiment Overview
| Repository ID: | FR-FCM-ZY6U | Experiment name: | Jung Eun Jang | MIFlowCyt score: | 19.50% |
| Primary researcher: | Jung Eun Jang | PI/manager: | Jung Eun Jang | Uploaded by: | Jung Eun Jang |
| Experiment dates: | 2015-01-21 - 2016-06-22 | Dataset uploaded: | May 2017 | Last updated: | May 2017 |
| Keywords: | None | Manuscripts: | |||
| Organizations: | None | ||||
| Purpose: | The purpose of the experiment presented here was to set up negative and positive gate strategies for the expression of IFN-gamma and granzyme B in CD4+Foxp3- and CD8 T cell population. | ||||
| Conclusion: | Naïve T cells stimulated with anti-CD3 and anti-CD28 and naïve T cells without stimulation were stained with anti-Granzyme B and anti-IFN-gamma antibodies as positive and negative control. We determined the gate of Granzyme B and IFN-gamma positive population based on negative and positive control. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | None | ||||
