Experiment Overview
| Repository ID: | FR-FCM-Z3E3 | Experiment name: | Cell cycle | MIFlowCyt score: | 64.75% |
| Primary researcher: | Chen Chen | PI/manager: | Chen Chen | Uploaded by: | Chen Chen |
| Experiment dates: | 2018-11-25 - 2018-12-06 | Dataset uploaded: | Jan 2021 | Last updated: | Jan 2021 |
| Keywords: | [cell cycle] | Manuscripts: | |||
| Organizations: |
Zhengzhou University, China, (Zhengzhou)
|
||||
| Purpose: | Knock down the fusion CTNNBIP1-CLSTN1, then detect whether this fusion can affect cell cycle. | ||||
| Conclusion: | Silencing CTNNBIP1-CLSTN1 resulted in cell cycle arrest in G2/M phase. | ||||
| Comments: | three cell lines. | ||||
| Funding: | Not disclosed | ||||
| Quality control: | use same untreated cells for quality control | ||||
Experiment variables
| Doses | |
|---|---|
| · 500 µl propidium iodide | Specimen_001_Tube_001_001 LO2NC.fcs · Specimen_001_Tube_002_002 293TNC.fcs · Specimen_001_Tube_002_002 LO2siC.fcs · Specimen_001_Tube_003_003 293TsiCC.fcs · Specimen_001_Tube_003_003 LO2siCC.fcs · Specimen_001_Tube_004_004 293TsiC.fcs |
| Timepoints | |
|---|---|
| · 60 minites | Specimen_001_Tube_001_001 LO2NC.fcs · Specimen_001_Tube_002_002 293TNC.fcs · Specimen_001_Tube_002_002 LO2siC.fcs · Specimen_001_Tube_003_003 293TsiCC.fcs · Specimen_001_Tube_003_003 LO2siCC.fcs · Specimen_001_Tube_004_004 293TsiC.fcs |
| Conditions | |
|---|---|
| · room temperature | Specimen_001_Tube_001_001 LO2NC.fcs · Specimen_001_Tube_002_002 293TNC.fcs · Specimen_001_Tube_002_002 LO2siC.fcs · Specimen_001_Tube_003_003 293TsiCC.fcs · Specimen_001_Tube_003_003 LO2siCC.fcs · Specimen_001_Tube_004_004 293TsiC.fcs |
| Sample Type | |
|---|---|
| · 293T cell lines | Specimen_001_Tube_002_002 293TNC.fcs · Specimen_001_Tube_003_003 293TsiCC.fcs · Specimen_001_Tube_004_004 293TsiC.fcs |
| · LO2 cell lines | Specimen_001_Tube_001_001 LO2NC.fcs · Specimen_001_Tube_002_002 LO2siC.fcs · Specimen_001_Tube_003_003 LO2siCC.fcs |
