Experiment Overview
| Repository ID: | FR-FCM-ZY86 | Experiment name: | Library 7 binding progression | MIFlowCyt score: | 90.00% |
| Primary researcher: | Jennifer Soundy | PI/manager: | Darren Day | Uploaded by: | Jennifer Soundy |
| Experiment dates: | 2015-09-10 - 2015-09-11 | Dataset uploaded: | Jul 2017 | Last updated: | Jul 2017 |
| Keywords: | [Bacteria] [Aptamer binding] [Pseudomonas aeruginosa] [SELEX progression] [FAM labelled aptamer] | Manuscripts: | |||
| Organizations: |
Victoria University of Wellington, School of Biological Sciences, Wellington, Wellington (New Zealand)
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| Purpose: | The purpose of this experiment was to check whether we were getting an increase in specificity in binding from our seventh library (L7) from SELEX compared to the library 0 (L0) starting library. Binding was assessed with Pseudomonas aeruginosa and E.coli to compare specific and non-specific associations. | ||||
| Conclusion: | We found that L7 was binding to P. aeruginosa more (shown as an increase in FAM fluorescence intensity) compared to the unselected starting library. We also found that L7 did not show increased binding to E.coli over L0, showing that the increase in binding is Pseudomonas specific. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | The same flow cytometer and settings are used for all experiments. The PBS used as flow buffer to re-suspend the bacteria in was run on its own to check for contamination or particulate that shows up as the same size as our target bacteria. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · P. aeruginosa | Aptamer samples_L0 pao1_005.fcs · Aptamer samples_L7 BF pao1_006.fcs · Cell conc_cell conc pao1_002.fcs |
| · E. coli | Aptamer samples_L0 DH5a_002.fcs · Aptamer samples_L7 BF DH5a_003.fcs · Cell conc_cell conc DH5a_001.fcs |
| Conditions | |
|---|---|
| · No aptamer | Cell conc_cell conc DH5a_001.fcs · Cell conc_cell conc pao1_002.fcs |
| · L0 library | Aptamer samples_L0 DH5a_002.fcs · Aptamer samples_L0 pao1_005.fcs |
| · L7 library | Aptamer samples_L7 BF DH5a_003.fcs · Aptamer samples_L7 BF pao1_006.fcs |
