Experiment Overview
| Repository ID: | FR-FCM-Z5VK | Experiment name: | Intracellular staining of Trastuzumab from the bulk cultures of GS-KO CHO-S cells | MIFlowCyt score: | 31.50% |
| Primary researcher: | Witsanu Srila | PI/manager: | Montarop Yamabhai | Uploaded by: | Witsanu Srila |
| Experiment dates: | 2020-03-02 - 2020-03-11 | Dataset uploaded: | Nov 2022 | Last updated: | Dec 2022 |
| Keywords: | [intracellular staining] [Trastuzumab] [GS-KO CHO-S cells] | Manuscripts: | |||
| Organizations: |
Suranaree University of Technology, Suranaree University of Technology, Mueng, Nakhon Ratchasima (Thailand)
University of Natural Resources and Life Sciences (BOKU), Biotechnology, wien, (Austria) |
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| Purpose: | Identify the expression strength of the monoclonal antibody and possible sub-populations of producer cells | ||||
| Conclusion: | Flow cytometry analysis by intracellular staining of heavy and light chains of Trastuzumab from the bulk cultures of each GS-KO CHO-S cell demonstrated that more than 98% of cells express both chains. | ||||
| Comments: | Flow cytometry dataset for Supplementary Figure S3 in manuscript | ||||
| Funding: | This research was supported by the Royal Golden Jubilee PhD program of Thailand [grant number PHD/0191/2557], Suranaree University of Technology, University of Natural Resources and Life Sciences (BOKU), and BIOTEC, National Science and Technology Development Agency (NSTDA) [grant number P-18-50127]. THis work was also supported by the Science Research and Innovation (TSRI) [grant no. RTA6180012] and Distinguished Research Professor Grant (NRCT 808/2563) of the National Research Council of Thailand. | ||||
| Quality control: | The experiments were performed in five biological replicates. Negative control was included. | ||||
