Experiment Overview
| Repository ID: | FR-FCM-Z7CG | Experiment name: | Cell cycle | MIFlowCyt score: | 51.50% |
| Primary researcher: | Drífa Guðmundsdóttir | PI/manager: | Stefan Sigurdsson | Uploaded by: | Drífa Guðmundsdóttir |
| Experiment dates: | 2018-10-02 - 2024-02-27 | Dataset uploaded: | Apr 2024 | Last updated: | Apr 2024 |
| Keywords: | [U2OS] [MITF] [Hippo pathway] | Manuscripts: | |||
| Organizations: |
University of Iceland, Reukjavik, (Iceland)
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| Purpose: | Cell cycle profile analysis after MITF, LATS1 or LATS2 knockdown in U2OS cells | ||||
| Conclusion: | MITF knockdown results in G1 cell cycle arrest in U2OS cells. This cell cycle arrest is rescued by a co-depletion of MITF and LATS2 but not with a co-depletion of MITF and LATS1. | ||||
| Comments: | None | ||||
| Funding: | Icelandic research fund and Icelandic cancer society research fund. | ||||
| Quality control: | Daily performance test of instrument. Compensation not required for assay. | ||||
Experiment variables
| Conditions | |
|---|---|
| · siCON | cc_siCON.fcs · cc_siCON.fcs-(1) · CC_siCON.fcs · cellcycle_mst1_yap_atr(1)_Group_siCON.fcs · DAPI siMITF-LATS1_Group_siCON.fcs · 7AAD staining(3)_Group_siRNA ctlr.fcs |
| · siMITF | cc_siMITF.fcs · cc_siMITF.fcs-(1) · CC_siMITF.fcs · cellcycle_mst1_yap_atr(1)_Group_siMITF.fcs · DAPI siMITF-LATS1_Group_siMITF.fcs · 7AAD staining(3)_Group_siMITF.fcs |
| · siLATS1 | cellcycle_mst1_yap_atr(1)_Group_siLATS1.fcs · DAPI siMITF-LATS1_Group_siLATS1.fcs · cc_siLATS1.fcs |
| · siMITF_siLATS1 | cellcycle_mst1_yap_atr(1)_Group_siMITF_LATS1.fcs · DAPI siMITF-LATS1_Group_siMITF-LATS1.fcs · cc_MITF_LATS1.fcs |
| · unstained | DAPI siMITF-LATS1_Group_UC.fcs · 7AAD staining(3)_Group_UC.fcs |
| · siLATS2 | cellcycle_mst1_yap_atr(1)_Group_siLATS2.fcs · CC_siLATS2.fcs · 7AAD staining(3)_Group_siLATS2.fcs |
| · siMITF_LATS2 | cellcycle_mst1_yap_atr(1)_Group_siMITF_LATS2.fcs · CC_siMITF_siLATS2.fcs · 7AAD staining(3)_Group_siMITF+LATS2.fcs |
