Experiment Overview
| Repository ID: | FR-FCM-Z5FT | Experiment name: | M1 vs M2 lung macrophages | MIFlowCyt score: | 50.00% |
| Primary researcher: | Maud Deny | PI/manager: | Maud Deny | Uploaded by: | Maud Deny |
| Experiment dates: | 2020-04-14 - | Dataset uploaded: | Jun 2022 | Last updated: | Jun 2022 |
| Keywords: | [mice] [GBS] [sex] [M1/M2 macrophages] | Manuscripts: | |||
| Organizations: |
Inflammation Unit, Laboratory of Pediatric Research, ULB, Brussels, (Belgium)
Immune Response Service, Sciensano, Brussels, (Belgium) |
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| Purpose: | This experiment was performed to compare the M1/M2 polarization of lung macrophages from 4-5 week old C57bl/6 mice naive or 24 hours after intranasal infection with 1.10^8 CFUs of group B streptococcus | ||||
| Conclusion: | The results showed a significant predominance of M1 phenotype, both at the percentage of positive cells and median fluorescence intensity (MFI) in male mice compared to female mice. | ||||
| Comments: | None | ||||
| Funding: | This research was supported by “IRIS research” | ||||
| Quality control: | Single color controls of each fluorochrome were run for compensation and FMO controls were run for gating | ||||
Experiment variables
| Conditions | |
|---|---|
| · Females GBS | 10. female GBS M1M2.fcs · 11 female GBS M1M2.fcs · 4. female GBS M1M2.fcs · 5. female GBS M1M2.fcs · 6 female GBS M1M2.fcs · 6. female GBS M1M2.fcs · 7 female GBS M1M2.fcs · 7 female GBS M1M2.fcs-(1) · 8 female GBS M1M2.fcs · 8. female GBS M1M2.fcs · 9 female GBS M1M2.fcs · 9. female GBS M1M2.fcs |
| · Males naives | 3 male naive M1M2.fcs · 4 male naive M1M2.fcs · 5 male naive M1M2.fcs · 5. male naive M1M2.fcs · 6 male naive M1M2.fcs |
| · Females naives | 10 female naive M1M2.fcs · 11. female naive M1M2.fcs · 15 female naive M1M2.fcs · 8 female naive M1M2.fcs · 9 female naive M1M2.fcs |
| · Males GBS | 1 male GBS M1M2.fcs · 1. male GBS M1M2.fcs · 1. male GBS M1M2.fcs-(1) · 2 male GBS M1M2.fcs · 2 male GBS M1M2.fcs-(1) · 2. male GBS M1M2.fcs · 2. male GBS M1M2.fcs-(1) · 3 male GBS M1M2.fcs · 3. male GBS M1M2.fcs · 3. male GBS M1M2.fcs-(1) · 4 male GBS M1M2.fcs · 4. male GBS M1M2.fcs · 5 male GBS M1M2.fcs |
