Experiment Overview
| Repository ID: | FR-FCM-Z4L6 | Experiment name: | Decoding Expression Dynamics of Protein and Transcriptome at the Single Cell Level through Multi-Omics Sequencing in Paired Picoliter Chambers | MIFlowCyt score: | 47.75% |
| Primary researcher: | Xing Xu | PI/manager: | Xing Xu | Uploaded by: | Xing Xu |
| Experiment dates: | 2019-08-01 - 2021-12-31 | Dataset uploaded: | Oct 2021 | Last updated: | Oct 2021 |
| Keywords: | [single-cell sequencing] [multi-omics analysis] | Manuscripts: | |||
| Organizations: |
Xiamen University, Xiamen University, Xiamen, (China)
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| Purpose: | To evaluate the protein expressions of MCF7, SK-BR-3 and MDA-MB-231 cells. | ||||
| Conclusion: | Simultaneous analysis of mRNAs and proteins at the single-cell level provides information about the dynamics and correlations of gene and protein expressions in individual cells, enabling comprehensive study of cellular heterogeneity and expression patterns. Here, we present a platform for high-throughput cellular indexing of mRNAs and proteins, named multi-Paired-seq, with high cell utilization, accurate molecular measurement and low cost. Based on hydrodynamic differential flow resistance, multi-Paired-seq largely improves cell utilization (>95%). Combined with pump/valve structure, cell-free antibodies and mRNAs can be removed completely for highly accurate detection. The picoliter reaction chambers allow higher detection sensitivity and lower sequencing cost. By using multi-Paired-seq, more elaborate classifications of breast cancers are identified according to multimodal measurements, and the expression correlations between mRNAs and proteins under altered conditions are quantified. Multi-Paired-seq provides multi-modal measurements at the single cell level, which offers a new tool for cell biology, developmental biology, drug discovery and precision medicine. | ||||
| Comments: | None | ||||
| Funding: | We thank the National Natural Science Foundation of China (21927806, 21735004, 21521004, 21325522, and 21904085), the National Key R&D Program of China (2018YFC1602900 and 2019YFA0905800), the Innovative Research Team of High-Level Local Universities in Shanghai, and the Program for Changjiang Scholars and Innovative Research Team in University (IRT13036) for their financial support. | ||||
| Quality control: | IgG protein for control measures | ||||
