Experiment Overview
| Repository ID: | FR-FCM-Z3E4 | Experiment name: | Cell apoptosis | MIFlowCyt score: | 64.75% |
| Primary researcher: | Chen Chen | PI/manager: | Chen Chen | Uploaded by: | Chen Chen |
| Experiment dates: | 2018-10-16 - 2018-11-25 | Dataset uploaded: | Jan 2021 | Last updated: | Jan 2021 |
| Keywords: | [APOPTOSIS] | Manuscripts: | |||
| Organizations: |
Zhengzhou university, Zhengzhou, (China)
|
||||
| Purpose: | Knock down the fusion CTNNBIP1-CLSTN1, then detect whether this fusion can affect cell apoptosis. | ||||
| Conclusion: | Silencing CTNNBIP1-CLSTN1 resulted in apoptosis | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | the same untreated cells were used as control. | ||||
Experiment variables
| Doses | |
|---|---|
| · 500 µl binding buffer containing 5 µl Annexin V-FITC and 5 µl PI stain | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_Tube_006_006nc LO2.fcs |
| · 500 µl binding buffer containing 5 µl Annexin V-FITC | Specimen_001_FITC_002 293T.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_FITC_002 LO2.fcs |
| · 500 µl binding buffer containing 5 µl PI | Specimen_001_PI_003 293T.fcs · Specimen_001_PI_003 HUVEC.fcs · Specimen_001_PI_003 LO2.fcs |
| Timepoints | |
|---|---|
| · 15 minutes | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_Tube_006_006nc LO2.fcs · Specimen_001_FITC_002 293T.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_FITC_002 LO2.fcs · Specimen_001_PI_003 293T.fcs · Specimen_001_PI_003 HUVEC.fcs · Specimen_001_PI_003 LO2.fcs |
| Sample Type | |
|---|---|
| · LO2 cell line | Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006nc LO2.fcs · Specimen_001_FITC_002 LO2.fcs · Specimen_001_PI_003 LO2.fcs |
| · 293T cell line | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_FITC_002 293T.fcs · Specimen_001_PI_003 293T.fcs |
| · HUVEC cell line | Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_PI_003 HUVEC.fcs |
| Conditions | |
|---|---|
| · room temperature | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_Tube_006_006nc LO2.fcs · Specimen_001_FITC_002 293T.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_FITC_002 LO2.fcs · Specimen_001_PI_003 293T.fcs · Specimen_001_PI_003 HUVEC.fcs · Specimen_001_PI_003 LO2.fcs |
