Experiment Overview
Repository ID: | FR-FCM-Z3E4 | Experiment name: | Cell apoptosis | MIFlowCyt score: | 64.75% |
Primary researcher: | Chen Chen | PI/manager: | Chen Chen | Uploaded by: | Chen Chen |
Experiment dates: | 2018-10-16 - 2018-11-25 | Dataset uploaded: | Jan 2021 | Last updated: | Jan 2021 |
Keywords: | [APOPTOSIS] | Manuscripts: | |||
Organizations: |
Zhengzhou university, Zhengzhou, (China)
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Purpose: | Knock down the fusion CTNNBIP1-CLSTN1, then detect whether this fusion can affect cell apoptosis. | ||||
Conclusion: | Silencing CTNNBIP1-CLSTN1 resulted in apoptosis | ||||
Comments: | None | ||||
Funding: | Not disclosed | ||||
Quality control: | the same untreated cells were used as control. |
Experiment variables
Doses | |
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· 500 µl binding buffer containing 5 µl Annexin V-FITC and 5 µl PI stain | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_Tube_006_006nc LO2.fcs |
· 500 µl binding buffer containing 5 µl Annexin V-FITC | Specimen_001_FITC_002 293T.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_FITC_002 LO2.fcs |
· 500 µl binding buffer containing 5 µl PI | Specimen_001_PI_003 293T.fcs · Specimen_001_PI_003 HUVEC.fcs · Specimen_001_PI_003 LO2.fcs |
Timepoints | |
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· 15 minutes | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_Tube_006_006nc LO2.fcs · Specimen_001_FITC_002 293T.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_FITC_002 LO2.fcs · Specimen_001_PI_003 293T.fcs · Specimen_001_PI_003 HUVEC.fcs · Specimen_001_PI_003 LO2.fcs |
Sample Type | |
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· LO2 cell line | Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006nc LO2.fcs · Specimen_001_FITC_002 LO2.fcs · Specimen_001_PI_003 LO2.fcs |
· 293T cell line | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_FITC_002 293T.fcs · Specimen_001_PI_003 293T.fcs |
· HUVEC cell line | Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_PI_003 HUVEC.fcs |
Conditions | |
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· room temperature | Specimen_001_Tube_004_004 293Tsicc.fcs · Specimen_001_Tube_004_004SICC HUVEC.fcs · Specimen_001_Tube_004_004sicc LO2.fcs · Specimen_001_Tube_005_005 293Tsic.fcs · Specimen_001_Tube_005_005NC HUVEC.fcs · Specimen_001_Tube_005_005sic LO2.fcs · Specimen_001_Tube_006_006 293Tnc.fcs · Specimen_001_Tube_006_006SIC HUVEC.fcs · Specimen_001_Tube_006_006nc LO2.fcs · Specimen_001_FITC_002 293T.fcs · Specimen_001_FITC_002 HUVEC.fcs · Specimen_001_FITC_002 LO2.fcs · Specimen_001_PI_003 293T.fcs · Specimen_001_PI_003 HUVEC.fcs · Specimen_001_PI_003 LO2.fcs |