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Experiment Overview

Repository ID: FR-FCM-ZZQP Experiment name: OMIP-036: Coinhibitory Receptors MIFlowCyt score: 16.50%
Primary researcher: Zachary Healy PI/manager: Zachary Healy Uploaded by: Zachary Healy
Experiment dates: 2016-04-13 - 2016-04-13 Dataset uploaded: Apr 2016 Last updated: Sep 2016
Keywords: None Manuscripts: Cytalogo
Organizations: None
Purpose: This panel was optimized to quantify inhibitory receptor expression on CD4 and CD8 T cells from differentiation and activation subsets (Table 1). Six inhibitory (i.e., immune checkpoint) receptors (PD-1, TIM-3, LAG-3, CD160, BTLA, CTLA-4) were chosen based upon previously published observations suggesting their role in modulating CD4 and CD8 T cell activation in response to persistent antigen exposure [1-3]. Furthermore, given the important observations that inhibitory receptor expression varies by differentiation and prior antigen experience, markers of T cell differentiation and prior antigen experience (CCR7, CD45RA, CD127, CD28, KLRG1) were also included [4-6]. This panel was developed and optimized for use in cryopreserved human peripheral blood mononuclear cells (PMBCs), although it has also been applied in fresh PBMCs other bodily fluids (e.g., malignant ascites).
Conclusion: See Text of paper
Comments: See text of paper
Funding: This publication was supported by R21 AI 099784-01A1, T32 HL007538, the Duke University Center for AIDS Research (CFAR) (P30 AI 64518) as well as by the National Center for Advancing Translational Sciences (NIH) (UL1TR001117). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Quality control: See text of paper
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