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Repository ID:
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FR-FCM-Z2CX
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Experiment name:
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Ma et al CD8+ T cell signalling panel experiment 1
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MIFlowCyt score:
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82.83%
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Primary researcher:
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Claire Ma |
PI/manager:
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Arianne Richard |
Uploaded by:
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Arianne Richard |
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Experiment dates:
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2018-10-01 - 2018-10-02 |
Dataset uploaded:
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Nov 2019
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Last updated:
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Nov 2019
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Keywords:
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[mass cytometry]
[CyTOF]
[CD8+ T cells]
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Manuscripts:
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[32412411]
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Organizations:
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Cambridge Institute for Medical Research, Department of Medicine, Cambridge, (UK)
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Purpose:
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We wished to understand how stimulation strength impacted the signalling pathways initiated downstream of TCR ligation. We stimulated OTI Rag1-deficient CD8+ T cells with 4 peptides (SIINFEKL (N4) = high potency, SIITFEKL (T4) = medium potency, SIIGFEKL (G4) = low potency, ASNENMDAM (NP68) = null) for 0, 1, or 2 hours and then examined their signalling using a custom mass cytometry panel. |
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Conclusion:
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We find that it is the rate of activation and not the organization of this programme that responds to signal strength.
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Comments:
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None
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Funding:
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This work was funded by the Wellcome Trust (grants [103930], [100140] and [217100] to G.M.G and grant 204017/Z/16/Z to C.Y.M); Cancer Research UK (core funding [A17197] to J.C.M); EMBL (core funding to J.C.M); the MRC (MR/P014178/1 to A.C.R); the ACT (grant [23/17 A (ii)] to C.Y.M). A.C.R also received a supplementary grant from the National Institute for Health Research [Cambridge Biomedical Research Centre at the Cambridge University Hospitals NHS Foundation Trust].
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Quality control:
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None |
